Cytotoxicity and oxidative stress study in cultured rat Sertoli cells with methyl tert-butyl ether (MTBE) exposure

Reproductive Toxicology
Dongmei LiXiaodong Han

Abstract

Cultured Sertoli cells were tested for their cytotoxicity and oxidative stress induced by methyl tert-butyl ether (MTBE) which has been extensively used as a gasoline additive. In cytotoxic experiments, Sertoli cells were cultured with medium alone (control), 5, 500, or 50,000 microM MTBE. Lactate dehydrogcnase (LDH) leakage assay, staining with fluorescein diacetate (FDA) and propidium iodide (PI), and flow cytometric analyses were used. In oxidative stress experiments, Sertoli cells were cultured with medium alone (control), 0.5, 50, or 5000 microM MTBE. The production of reactive oxygen species (ROS), maleic dialdehyde (MDA) content and the level of superoxide dismutase (SOD) activity in cell supernatants were measured. Meanwhile, the expression level of 8-oxoguanine DNA glycosidase (OGG1) and extracellular form of superoxide dismutase (SOD(EX)) in Sertoli cells were determined by RT-PCR. We also compared the current findings with the previous findings in rat spermatogenic cells exposed to MTBE. The present data indicate that high dose MTBE may exert a direct toxic effect on Sertoli cells. Oxidative stress induced by MTBE is a possible mechanism of cytotoxicity.

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Citations

Sep 10, 2014·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Yang HuDongmei Li
Jan 23, 2016·Environmental Science and Pollution Research International·Ahmad SalimiJalal Pourahmad
Dec 19, 2015·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Arastoo SaeediAfshin Mohammadi-Bardbori
Feb 6, 2010·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Chunqi Wu
Sep 21, 2013·Toxicon : Official Journal of the International Society on Toxinology·Yu ChenXiaodong Han
Dec 27, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yang HuXiao-Dong Han
May 13, 2014·Regulatory Toxicology and Pharmacology : RTP·Ann de Peyster, Ellen Mihaich
Feb 6, 2017·The Journal of Physical Chemistry. a·Matthew WinfoughGiovanni Meloni

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