Cytotoxicity and transformation of C3H10T1/2 cells induced by areca nut components

Journal of the Formosan Medical Association = Taiwan Yi Zhi
Chien-Yang YehJiiang-Huei Jeng

Abstract

Betel quid (BQ) chewing is popular in Taiwan and many other countries. There are about 200-600 million BQ chewers in the world. BQ chewing is one major risk factor of oral cancer and oral submucous fibrosis (OSF). While areca nut (AN), a main component of BQ, exhibits genotoxicity, its transformation capacity and its role in the initiation and promotion stages of carcinogenesis are not fully clear. Mouse C3H10T1/2 cells were exposed to AN extract (ANE) for 24 hours. Cytotoxicity was evaluated by colony forming efficiency. For the transformation assay, C3H10T1/2 cells were exposed to ANE for 24 hours and then incubated in medium with/without 12-O-tetradecanolylphorbol-13-acetate (TPA; a tumor promoter) for 42 days. Cells were stained with Giemsa and type II and type III transformed foci were counted for analysis of the transformation capacity of ANE. ANE exhibited cytotoxicity to C3H10T/12 cells at concentrations higher than 320 μg/mL as shown by a decrease in colony numbers. ANE (80-640 μg/mL) alone mildly stimulated the transformed foci formation (p > 0.05). In the presence of TPA, ANE (80-640 μg/mL) markedly stimulated the transformed foci formation. The percentage of dishes with foci increased from 0% in controls to 20% in A...Continue Reading

References

Jul 1, 1992·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Y C KoS F Hsieh
Dec 1, 1993·Environmental Health Perspectives·B G Short
Apr 1, 1993·Environmental Health Perspectives·J C Barrett
Nov 1, 1995·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Y C KoC C Tsai
Sep 1, 1996·European Journal of Cancer. Part B, Oral Oncology·Y T JinR M Chen
Feb 24, 2001·Human & Experimental Toxicology·J E KlaunigY Xu
Dec 12, 2001·Oral Microbiology and Immunology·L J LingY L Lai
Sep 10, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ming-Hsun LinChau-Jong Wang
Oct 25, 2003·Archives of Toxicology·Ming-Hsun LinFen-Pi Chou
Jan 22, 2008·Archives of Toxicology·Christoph KöhleKarl Walter Bock

❮ Previous
Next ❯

Citations

Sep 29, 2015·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Chien-Yang YehJiiang-Huei Jeng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.