Cytotoxicity assessment of gliotoxin and penicillic acid in Tetrahymena pyriformis

Environmental Toxicology
Carolin GräbschA Müller

Abstract

Various studies have documented the associations between mold exposure and effects on health. Mycotoxins, which occur in spores and mold fragments, can be involved in processes that have pathological effects, such as adynamia of the immune system, recurrent infections of the respiratory tract, or asthma. Using Tetrahymena pyriformis, a single-cell organism well established as a suitable model for human respiratory epithelium-cell functionalities, we investigated dose-response relationships of the mycotoxins gliotoxin and penicillic acid. Our study focused on the viability (cell count, MTT assay), energy levels (adenosine-5'-triphosphate content), energy-providing processes (MTT reduction per cell), and cell respiration (oxygen consumption). Both mycotoxins acted as cytotoxins in a dose-dependent manner. Gliotoxin had a stronger inhibitory effect (EC50 0.38 microM) than did penicillic acid (EC50 343.19 microM). The energy-providing processes were not inhibited or were only weakly inhibited under the influence of gliotoxin, whereas penicillic acid caused stimulation of the physiological parameters. Summarizing the results, it is clear that the two investigated mycotoxins must have different modes of action. They are not only diff...Continue Reading

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Citations

Jul 16, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A MuellerO Herbarth
Feb 28, 2016·Applied and Environmental Microbiology·Hana TriguiSébastien P Faucher
Nov 30, 2019·Mycotoxin Research·Birgitte AndersenRobert A Eustace

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