Cytotoxicity comparison of three current direct pulp-capping agents with a new bioceramic root repair putty

Journal of Endodontics
Wade R HirschmanMichael M Hoen

Abstract

The purpose of this in vitro study was to compare the cytotoxicity of white mineral trioxide aggregate cement (AMTA, MTA-Angelus), Brasseler Endosequence Root Repair Putty (ERRM), Dycal, and Ultra-blend Plus (UBP) by using human dermal fibroblasts and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cultured adult human dermal fibroblasts were exposed to multiple concentrations of material elutes. The test material samples were immersed and incubated in the culture medium for 2, 5, or 8 days at 37°C. The cytotoxic effects were recorded by using an MTT-based colorimetric assay. Positive and negative controls were used. The results were statistically examined by one-way analysis of variance and Tukey post tests. The cell viability of cultures exposed to all dilutions of AMTA, ERRM, and UBP was statistically similar to the negative control at 2 and 5 days. Only the Dycal-exposed specimens exhibited a statistically significant increase in cytotoxicity at the 2 initial evaluation periods. After exposure to the 8-day elutes, the respective percentage of cell survivability was 91% (Brasseler), 88% (MTA-Angelus), 76% (Ultra-blend Plus), and 37% (Dycal). From the data in this in vitro study, AMTA, ERRM, and UB...Continue Reading

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Citations

Apr 12, 2014·ISRN Dentistry·Aniket S WadajkarTakashi Komabayashi
Aug 21, 2013·Journal of Endodontics·Keisuke HandaTakashi Saito
Aug 7, 2013·The Veterinary Clinics of North America. Equine Practice·Jennifer T Rawlinson, Edward Earley
Mar 26, 2013·Journal of Endodontics·Hui-min ZhouMarkus Haapasalo
Feb 14, 2013·Journal of Endodontics·Sin-Yeon ChoIl-Young Jung
Nov 14, 2012·Journal of Endodontics·Bin-Na LeeWon-Mann Oh
Jul 17, 2012·Journal of Endodontics·Mahmoud Reza ModareszadehNarges Salamat
Oct 6, 2015·Journal of Investigative and Clinical Dentistry·Alaa E DawoodDavid J Manton
Mar 22, 2014·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Nawaf LabbanJeffrey A Platt
Feb 1, 2015·Journal of Endodontics·Siyi LiuYanmei Dong
May 8, 2013·Archives of Oral Biology·Ines WillershausenBenjamin Briseño Marroquin
May 8, 2016·Clinical Oral Investigations·Xin LiBart Van Meerbeek
Jun 18, 2014·Journal of Endodontics·Ryan M WalshJianing He
Jan 22, 2017·Journal of Materials Science. Materials in Medicine·Ali Abedi-AminSalvatore Sauro
Mar 16, 2017·Journal of Endodontics·Pierre Schembri-Wismayer, Josette Camilleri
Apr 9, 2016·Brazilian Dental Journal·Hernán Coaguila-LlerenaZulema Velásquez-Huamán
Aug 20, 2015·Journal of Dental Research·A N MooreJ S Colombo
Aug 12, 2014·Restorative Dentistry & Endodontics·Sedigheh KhedmatPaul Michael Howell Dummer
Apr 9, 2019·European Journal of Oral Sciences·Mohamed R W AliAthanasia Bletsa
Jul 28, 2019·British Dental Journal·Sarah JadunJames Darcey
Feb 11, 2020·Journal of International Society of Preventive & Community Dentistry·Nasrin Mahgoub Tao Hong
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Nov 22, 2020·Journal of Dentistry·Qin SunFranklin R Tay
Jun 15, 2021·Restorative Dentistry & Endodontics·Francine BenettiLuciano Tavares Angelo Cintra
Jul 31, 2021·International Journal of Clinical Pediatric Dentistry·Viral MaruRupanshi Parekh

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