Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis

Dental Materials Journal
Meral Arslan MalkoçSema Sezgin Hakki

Abstract

To evaluate the cytotoxicity of resin cements on dental pulp-derived cells (bDPCs), Bifix QM (BQM), Choice 2(C2), RelyX U200(RU200), Maxcem Elite(ME), and Multilink Automix(MA) were tested. The materials were incubated in DMEM for 72 h. A real-time cell analyzer was used to evaluate cell survival. The statistical analyses used were one-way ANOVA and Tukey-Kramer tests. BQM, RU200, and ME demonstrated a significant decrease in the bDPCs' index at 24 and 72 h (p≤0.001). These materials were found to be the most toxic resin cements, as compared to the control and other tested materials (C2 and MA). However, C2 and MA showed a better survival rate, compared to BQM, RU200, and ME, and had lower cell index than the control group. The cytotoxic effects of resin cements on pulpa should be evaluated during the selection of proper cements.

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Citations

Oct 27, 2015·Microscopy Research and Technique·Osvaldo L BezzonLéa A B Silva
Oct 23, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·C KerezoudiG Palaghias
Jun 9, 2020·Dental Materials Journal·Gülbike DemirelÖzgür Irmak
Oct 1, 2019·Dental Materials Journal·Gülbike DemirelMehmet Ali Kiliçarslan
May 8, 2020·The Journal of Advanced Prosthodontics·Soner ŞişmanoğluNeslihan Tekce

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