Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay.

Journal of Applied Oral Science : Revista FOB
Gabriela Tavares Bekner CorreaMiriam F Zaccaro Scelza

Abstract

The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level. Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (p<0.05). Ten-fold dilutions of L&C, Fill Canal and AH Plus killed 24, 35 and 61% of THP-1 cells (p<0.05), respectively. Dilutions lesser than 1% caused minimal cell death as compared to the control groups (p>0.05), except for L&C 1% extract. The results revealed that the L&C paste crude extract was less cytotoxic to THP-1 cells than AH Plus or Fill Canal crude extracts.

References

Oct 1, 1989·Journal of Endodontics·K E SafaviG Sapounas
Apr 1, 1986·Journal of Endodontics·H NakamuraM Yip
Jul 1, 1974·Mechanisms of Ageing and Development·C F Van BezooijenD L Knook
Apr 29, 1999·Endodontics & Dental Traumatology·M R LeonardoL S Utrilla
Feb 24, 2001·Journal of Endodontics·N G AzarF Shokri
Jul 27, 2001·Journal of Endodontics·B WillershausenR Schulze
Oct 26, 2002·Journal of Endodontics·Kwang-Won LeeDavid H Pashley
Dec 10, 2002·Journal of Endodontics·T SchwarzeW Geurtsen
Jun 6, 2003·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Robert HaglundQiang Zhu
Dec 1, 1964·Oral Surgery, Oral Medicine, and Oral Pathology·H M RAPPAPORTP KAPSIMALIS
Aug 22, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Miriam F Zaccaro ScelzaSuzana Côrte-Real Faria

❮ Previous
Next ❯

Citations

Feb 19, 2016·Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research·Jacy Simi JuniorJoão Humberto Antoniazzi
Sep 1, 2015·Clinical Oral Investigations·Gleyce O SilvaCarlos H R Camargo
Jul 30, 2016·The Journal of Clinical Pediatric Dentistry·Maria Escobar-GarcíaAmaury Pozos-Guillén
Mar 17, 2017·Brazilian Dental Journal·Leticia Boldrin MestieriMário Tanomaru-Filho
Apr 6, 2013·Journal of Applied Oral Science : Revista FOB·Emmanuel João Nogueira Leal da SilvaAlexandre Augusto Zaia
Apr 9, 2016·Brazilian Dental Journal·Raquel Assed Bezerra SegatoLéa A B da Silva
Dec 16, 2020·Nanomedicine : Nanotechnology, Biology, and Medicine·Xin HuangBingdi Chen

❮ Previous
Next ❯

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.