Cytotoxicity induced by exposure to natural and synthetic tremolite asbestos: an in vitro pilot study

Acta histochemica
Armanda PugnaloniElena Belluso

Abstract

Mineral fibers are potential carcinogens to humans. In order to help clarify the etiology of the pathological effects of asbestos, cellular reactions to natural and synthetic asbestos fibers were compared using a lung alveolar cancer cell line (A549 epithelial cells), considered the first target of inhaled micro-environmental contaminants. Natural asbestos tremolite (NAT) fibers were collected from rocks in NW Italy. Synthetic asbestos tremolite (SAT) was iron-free and therefore considered as standard tremolite. Both fibers, subjected to mineralogical characterization by X-ray powder diffractometry, electron microscopy and energy dispersive spectrometry, fell within the definition of respirable and potentially carcinogenic fibers. Several signs of functional and structural cell damage were found after treatment with both fibers, documented by viability, motility, and morphological perturbations. Phalloidin labeling showed irregular distribution of cytoskeletal F-actin, whereas immunohistochemical investigations showed abnormal expression of VEGF, Cdc42, β-catenin, assessed as risks indicators for cancer development. Both fibers caused significant loss of viability, even compared to UICC crocidolite, but, while SAT fibers exerte...Continue Reading

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Citations

Dec 4, 2014·Acta histochemica·Armanda PugnaloniAlessandro F Gualtieri
Jun 13, 2016·Environmental Pollution·Andrea BloiseElena Belluso
Jul 8, 2017·Environmental Science and Pollution Research International·Yan CuiQingbi Zhang
Mar 23, 2018·International Journal of Environmental Health Research·Antonella CampopianoSergio Iavicoli
Oct 25, 2020·Environmental Geochemistry and Health·Ítalo L MontofréMaría E Gálvez
Jul 28, 2020·The Science of the Total Environment·Antonella CampopianoSergio Iavicoli
Jan 22, 2021·Environmental Geochemistry and Health·Andrea BloiseDolores Pereira

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