Cytotoxicity of 2,2',3,5',6-Pentachlorobiphenyl (PCB95) and its metabolites in the chicken embryo liver cells of laying hens

Ecotoxicology and Environmental Safety
Guangqin LiaoRuyan Hou

Abstract

2,2',3,5',6-Pentachlorobiphenyl (PCB95) is known as a persistent pollutant that was found in eggs in China. PCB 95 can be metabolized into OH-PCB95 and MeO-PCB95 in liver microsomes. However, the toxicity and its mechanism of PCB95 or its metabolites have been little studied on laying hens. Herein, chicken embryo liver cells of laying hens were selected and treated with different levels of PCB95 and its two metabolites, and the EC50 of PCB95, OH-PCB95, MeO-PCB95 was 80.85, 4.81 and 107.04 μg/mL respectively, indicating that OH-PCB95 is much more cytotoxic than PCB95 or MeO-PCB95. Targeted metabolomics was further used to study the effects of the parent compound and its metabolites on cell metabolism. The results showed that four primary types of glycerophospholipids were down-regulated after exposure to PCB95 and its metabolites, especially PE and PS (60% more than the control for PCB95, 40% for OH-PCB95, and less than 40% for MeO-PCB95). KEGG pathway analysis based on amino acid metabolism showed that PCB95 may mainly interfere with the amino acids involved in immune regulation (phenylalanine and tyrosine), and OH-PCB95 may be associated with genetic disoders (cysteine, methionine and purine metabolism). However, the metabolic...Continue Reading

References

Sep 1, 1975·Journal of Agricultural and Food Chemistry·S SafeD Jones
Jan 1, 1990·Critical Reviews in Toxicology·E M SilberhornL W Robertson
Oct 20, 1999·Archives of Biochemistry and Biophysics·J M JacobsJ F Sinclair
Oct 22, 2002·Journal of Pharmacological and Toxicological Methods·Jeremy T GambleGerald S Marks
Jan 13, 2004·Applied Microbiology and Biotechnology·T A Müller, H-P E Kohler
May 18, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Caiqin Zhou, Caiqiao Zhang
Jul 25, 2006·Comptes rendus biologies·Dominique Langin
Feb 24, 2009·Free Radical Biology & Medicine·Wendy A SpencerRamesh C Gupta
Apr 29, 2009·Colloids and Surfaces. B, Biointerfaces·Katarzyna Hac-WydroPatrycja Dynarowicz-Łatka
Nov 26, 2009·Pharmacology & Therapeutics·Isaac N PessahPamela J Lein
Aug 16, 2012·Translational Psychiatry·Y HeD Rujescu
Sep 15, 2012·Environmental Science & Technology·Izabela Kania-KorwelHans-Joachim Lehmler
Apr 19, 2013·Amino Acids·Guoyao Wu
Jul 16, 2013·Environmental Science and Pollution Research International·Katsunori Anezaki, Takeshi Nakano
Nov 28, 2013·Environment International·Xin HuPeter S Thorne
Jan 30, 2015·Critical Reviews in Toxicology·Fabian A GrimmLarry W Robertson
Feb 6, 2015·Environmental Science and Pollution Research International·Izabela Kania-Korwel, Hans-Joachim Lehmler
Apr 1, 2015·Environmental Science and Pollution Research International·Izabela Kania-Korwel, Hans-Joachim Lehmler
Jan 26, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Karyne BellehumeurThomas W Moon
Mar 9, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Huiyuan ZhaoXiaozhe Zhang

❮ Previous
Next ❯

Citations

Apr 5, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tiancai WangYongzhong Qian
May 15, 2021·Ecotoxicology and Environmental Safety·Lei YangZhaobin Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.