Cytotoxicity of albebetin oligomers depends on cross-beta-sheet formation

FEBS Letters
V V ZamotinLudmilla A Morozova-Roche

Abstract

Prefibrillar cytotoxicity was suggested as a common amyloid characteristic. We showed two types of albebetin prefibrillar oligomers are formed during incubation at pH 7.3. Initial round-shaped oligomers consist of 10-15 molecules determined by atomic force microscopy, do not bind thioflavin-T and do not affect viability of granular neurons and SH-SY5Y cells. They are converted into ca. 30-40-mers possessing cross-beta-sheet and reducing viability of neuronal cells. Neither monomers nor fibrils possess cytotoxicity. We suggest that oligomeric size is important for stabilising cross-beta-sheet core critical for cytotoxicity. As albebetin was used as a carrier-protein for drug delivery, examination of amyloidogenicity is required prior polypeptide biomedical applications.

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Citations

Nov 13, 2013·European Biophysics Journal : EBJ·Mohd Shahnawaz KhanJaved Musarrat
Sep 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Angel OrteDavid Klenerman
Jan 10, 2009·BMC Biotechnology·Chun-Yung HuangShu-Mei Liang
Dec 1, 2006·Journal of Molecular Biology·Anna L GharibyanLudmilla A Morozova-Roche
Sep 29, 2009·Biochimica Et Biophysica Acta·Valeria VetriValeria Militello
Nov 17, 2009·Journal of Molecular Biology·Mantas MalisauskasLudmilla Morozova-Roche
Oct 12, 2011·Journal of Molecular Biology·Xueen JiaLudmilla A Morozova-Roche
Jul 10, 2008·Investigational New Drugs·Noor Al-DasooqiDorothy M Keefe
Feb 9, 2020·Biomolecules·Vitalii BalobanovMikhail Kirpichnikov
Mar 27, 2009·Molecular and Cellular Biochemistry·Nitin Chaudhary, Ramakrishnan Nagaraj

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