Cytotoxicity of ambient air particles to rat lung macrophages: Comparison of cellular and functional assays

Toxicology in Vitro : an International Journal Published in Association with BIBRA
D NadeauA Dufresne

Abstract

The biological reactivity of ambient air particles was studied in five in vitro lung macrophage assays, involving the release of cytoplasmic and lysosomal enzymes, cellular ATP, neutral red uptake, tetrazolium reduction, and chemiluminescence. Macrophages from rat lungs (2 x 10(5) cells; 1 cm(2) attachment surface; 1 ml culture medium) were exposed for 18 hr to 0-100 mug of (1) the urban dust SRM 1649, (2) titanium dioxide (TiO(2)) or (3) DQ-12 quartz. On the basis of the depressions of neutral red uptake and cellular ATP, and the extracellular releases of lactate dehydrogenase, acid phosphatase and beta-glucuronidase, the ranking of cytotoxicity was as follows: quartz (EC(50) = 20-60 mug/ml) > > SRM 1649 approximately TiO(2) (EC(50) > 100mug/ml). The decrease in 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction was more sensitive to effects of the urban dust, with an EC(50) value for SRM 1649 (35mug/ml) intermediate between those for quartz (15mug/ml) and TiO(2) (82mug/ml). Although SRM 1649 could affect mitochondrial function, the impact of the urban dust on cellular integrity after 18 hr was comparable to that of TiO(2) particles. In contrast, SRM 1649 had profound effects on phagocytosi...Continue Reading

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Citations

Jun 27, 2015·Mutagenesis·Audrey F Dumax-VorzetAndrew C Povey
Mar 8, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Dalibor BreznanRenaud Vincent
Oct 7, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Dalibor BreznanRenaud Vincent
Apr 12, 2001·Toxicology and Applied Pharmacology·L C RenwickA Clouter
Jul 18, 2000·Toxicology and Applied Pharmacology·I Y AdamsonR Vincent
Dec 10, 1998·The American Journal of Pathology·L BouthillierP Kumarathasan
Nov 6, 2002·Environmental Health Perspectives·Estelle FachPrabir K Dutta
Oct 29, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·H MukaeS F van Eeden
Oct 18, 2018·Inhalation Toxicology·Matthew S P BoylesVicki Stone
Feb 2, 2010·Journal of Pharmacological and Toxicological Methods·Shahnaz Bakand, Amanda Hayes

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