Cytotoxicity of berberine on human cervical carcinoma HeLa cells through mitochondria, death receptor and MAPK pathways, and in-silico drug-target prediction

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Binan LuJinyong Peng

Abstract

Berberine, a natural product, has been widely used to treat hyperlipoidemia and intestinal diseases. In the present paper, berberine showed a significant anti-proliferative effect to human cervical carcinoma HeLa cells confirmed by 3-(4,5)-dimethyl-thiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT), flow cytometry analysis (FCM) and so on. The methods including western blotting, radioimmunity assay (RIA), reverse transcription-polymerase chain reaction (RT-PCR) were used to investigate protein and mRNA expressions. We found that Bcl-2/Bax ratio was significantly decreased and cytochrome c was released from mitochondrion to cytosol, which indicated that the mitochondrial pathway was activated by berberine. The up-regulation of Fas, FasL, TNF-alpha and TRAF-1 indicated the involvement of the death receptor pathway in the process of berberine-induced apoptosis. Furthermore caspase-3 and caspase-8 were activated as a central event of apoptosis, and the levels of phosphorylation of mitogen-activated protein kinases (MAPKs) were also investigated. In addition, the increased expression of p53 was also observed in berberine-treated HeLa cells, and as a node point of these different pathways in a protein-protein interaction network co...Continue Reading

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Citations

Jun 29, 2014·European Journal of Pharmacology·Parham Jabbarzadeh KaboliKing-Hwa Ling
Apr 23, 2013·Journal of Natural Medicines·Yuchen CaiWenqi Jiang
May 24, 2012·European Journal of Pharmacology·Kotamballi N Chidambara MurthyBhimanagouda S Patil
May 18, 2016·Expert Opinion on Drug Discovery·Aeri LeeDongsup Kim
Aug 21, 2018·Journal of Cellular Biochemistry·AmirReza HesariAmirhossein Sahebkar
Dec 3, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Mei LiZu-Bin Zhang
Jun 6, 2018·Frontiers in Chemistry·Hongbin HuangZunnan Huang
Sep 8, 2016·Molecular Medicine Reports·Hai-Yan WangYu-Lan Zheng
Oct 22, 2020·Critical Reviews in Food Science and Nutrition·Ankita KumariRajeev Kapila
Nov 18, 2020·Toxins·Anna OchRenata Nowak
Jan 12, 2021·Saudi Journal of Biological Sciences·Dilsad Onbasli, Gokcen Yuvali

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