Cytotoxicity of corrosion products of degradable Fe-based stents: relevance of pH and insoluble products

Colloids and Surfaces. B, Biointerfaces
Natalia S FagaliMónica A Fernández Lorenzo de Mele

Abstract

Fe-based biodegradable metallic materials (Fe-BMMs) have been proposed for cardiovascular applications and are expected to disappear via corrosion after an appropriate period. However, in vivo studies showed that Fe ions release leads to accumulation of orange and brownish insoluble products at the biomaterial/cell interface. As an additional consequence, sharp changes in pH may affect the biocompatibility of these materials. In the present work, the experimental protocols were designed with the aim of evaluating the relative importance that these factors have on biocompatibility evaluation of BMMs. Mitochondrial activity (MTT assay) and thiobarbituric acid reactive substances (TBARS) assay on mammalian cells, exposed to 1-5 mM of added Fe3+ salt, were assessed and compared with results linked exclusively to pH effects. Soluble Fe concentration in culture medium and intracellular Fe content were also determined. The results showed that: (i) mitochondrial activity was affected by pH changes over the entire range of concentrations of added Fe3+ assayed, (ii) at the highest added Fe3+ concentrations (≥3 mM), precipitation was detected and the cells were able to incorporate the precipitate, that seems to be linked to cell damage, (...Continue Reading

References

Jan 1, 1978·Biochemical Pharmacology·Y Masuda, T Murano
Nov 1, 1995·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·A M SieuwertsJ A Foekens
Aug 6, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·A J García-FernándezD Ordóñez
Oct 6, 2005·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Peter ZartnerMichael Weyand
Apr 1, 2006·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Dietmar SchranzHakan Akintürk
Jun 13, 2006·Biomaterials·Matthias PeusterChristian von Schnakenburg
Oct 4, 2006·Annual Review of Physiology·Nancy C Andrews, Paul J Schmidt
Dec 19, 2007·Journal of Interventional Cardiology·Ron WaksmanFermin O Tio
Jan 24, 2009·Journal of Biomedical Materials Research. Part a·Ana L Di VirgilioMonica Fernández Lorenzo de Mele
Jun 17, 2009·Current Opinion in Gastroenterology·Gregory J AndersonGordon D McLaren
Apr 26, 2011·Toxicology Letters·Sunhapas SoodvilaiVaranuj Chatsudthipong
Jun 30, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ana L Di VirgilioMonica Fernández Lorenzo de Mele
Sep 10, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Daniel PiersonJeremy Goldman
Sep 13, 2012·Journal of Nanoscience and Nanotechnology·Dong-Wook HanJong-Chul Park
Mar 19, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Michael SchinhammerPeter J Uggowitzer
Apr 19, 2013·Journal of Biomedical Materials Research. Part a·Claudia A GrilloMónica A Fernández Lorenzo de Mele
Jul 23, 2013·Toxicology Letters·Xiumei JiangChristiane Beer
Jan 15, 2014·Materials Science & Engineering. C, Materials for Biological Applications·T HuangY F Zheng
Mar 29, 2014·Colloids and Surfaces. B, Biointerfaces·Claudia A GrilloMónica A Fernández Lorenzo de Mele
May 13, 2014·Pathology, Research and Practice·MingChang ZhangAllen Burke
Mar 1, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Michael SchinhammerPeter J Uggowitzer

❮ Previous
Next ❯

Citations

Mar 2, 2016·Colloids and Surfaces. B, Biointerfaces·Tao HuangYufeng Zheng
Dec 23, 2017·International Journal of Molecular Sciences·Ke YangXingdong Zhang
Sep 22, 2017·Interventional Medicine & Applied Science·Maksym PogorielovSergii Zhdanov
Jun 6, 2020·Scientific Reports·Bernd WegenerPeter Quadbeck
May 29, 2018·Innovative Surgical Sciences·Nicole Gabriele GrünAnnelie-Martina Weinberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinica Chimica Acta; International Journal of Clinical Chemistry
K KolanjiappanM Kayalvizhi
Iyō Kizai Kenkyūjo hōkoku. Reports of the Institute for Medical and Dental Engineering, Tokyo Medical and Dental University
C ZhangA Watanabe
© 2021 Meta ULC. All rights reserved