PMID: 11318043Apr 25, 2001Paper

Cytotoxicity of dental glass ionomers evaluated using dimethylthiazol diphenyltetrazolium and neutral red tests

Acta Odontologica Scandinavica
E C Lönnroth, J E Dahl

Abstract

The purpose of this study was to assess the cytotoxicity of some commonly used glass ionomers. Three chemically cured glass ionomers (Fuji II, Lining cement, and Ketac Silver) and one light-cured (Fuji II LC) were tested. Extracts of mixed non-polymerized materials and polymerized specimens were prepared in accordance with ISO standard 10993-12. The polymerized specimens were cured and placed either directly in the medium (freshly cured), left for 24 h (aged), or aged plus ground before being placed in the medium. The cytotoxicity of extracts was evaluated on mouse fibroblasts (L, 929), using dimethylthiazol diphenyltetrazolium (MTT) and neutral red (NR) assays. Further, the concentrations of aluminum, arsenic and lead were analyzed in aqueous extracts from freshly cured and aged samples, and the fluoride levels analyzed in aqueous extracts from freshly cured samples. All extracts except that of non-polymerized Ketac Silver were rated as severely cytotoxic in both assays. Extracts of polymerized material were significantly more cytotoxic than extracts of non-polymerized material. All freshly cured glass ionomers released aluminum and fluoride concentrations far above what is considered cytotoxic (aluminum >0.2 ppm and fluoride ...Continue Reading

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Apr 25, 2000·Acta Odontologica Scandinavica·G B Sletten, J E Dahl

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Citations

Jan 7, 2005·Clinical Oral Investigations·A S Al-HiyasatM M Milhem
Mar 25, 2016·European Journal of Dentistry·La-Ongthong Vajrabhaya, Suwanna Korsuwannawong
Apr 24, 2015·Dental Materials Journal·Rita Trumpaite-VanagieneVygandas Rutkunas
Mar 14, 2008·Dental Materials : Official Publication of the Academy of Dental Materials·Gökçe MeriçI Eystein Ruyter
Jul 10, 2014·Brazilian Oral Research·Rogério Lacerda dos SantosMatheus Melo Pithon
Jan 24, 2006·Acta Odontologica Scandinavica·Alexander FranzAndreas Schedle
Oct 30, 2003·Acta Odontologica Scandinavica·Jan W V van Dijken, Karin Sunnegårdh-Grönberg

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