Cytotoxicity of dentine bonding agents on human pulp cells is related to intracellular glutathione levels

International Endodontic Journal
F-M HuangY-C Chang

Abstract

To evaluate ex vivo the mechanisms of cytotoxicity of dentine bonding agents in human pulp cells in vitro. Human pulp cells were obtained from impacted third molars with informed consent and then cultured using an explant technique. Set specimens from Clearfil SE Bond (CB), Prime & Bond 2.1 (PB), and Single Bond (SB) were eluted with culture medium. Cytotoxicity was judged using an assay of tetrazolium bromide reduction. To determine whether glutathione (GSH) levels were important in the cytotoxicity of dentine bonding agents, cells were pretreated with 2-oxothiazolidine-4-carboxylic acid (OTZ) to boost GSH levels or buthionine sulfoximine (BSO) to deplete GSH. Three replicates of each dentine bonding agents were performed in each test. All assays were repeated three times to ensure reproducibility. Statistical analysis was by one-way analysis of variance (anova). Tests of differences of the treatments were analysed by Duncan's test. Clearfil SE Bond, PB, and SB were cytotoxic to pulp cells in a concentration-dependent manner (P<0.05). The cytotoxicity was upregulated by dentine bonding agents in the following order: PB>SB>CB. Addition of OTZ extracellularly protected the pulp cells from dentine bonding agents-induced cytotoxic...Continue Reading

References

Jan 1, 1978·International Review of Cytology·N S Kosower, E M Kosower
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J K Dethmers, A Meister
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J M WilliamsonA Meister
Sep 1, 1995·Journal of Dental Research·S RatanasathienJ B Dennison
Jul 1, 1994·Journal of Oral Rehabilitation·T M Gerzina, W R Hume
Jan 1, 1996·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·W R Hume, T M Gerzina
Apr 29, 1998·Clinical Oral Investigations·G Schmalz
Nov 11, 1999·Journal of Biomedical Materials Research·W GeurtsenG Leyhausen
Jun 23, 2000·Dental Materials : Official Publication of the Academy of Dental Materials·C A CostaC T Hanks
Oct 6, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·W Geurtsen
May 7, 2002·Dental Materials : Official Publication of the Academy of Dental Materials·B ThonemannH Schweikl
Nov 28, 2002·International Endodontic Journal·F-M Huang, Y-C Chang
Mar 17, 2004·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Fu-Mei HuangYu-Chao Chang
Mar 18, 2004·Journal of Oral Rehabilitation·Peter Svensson, Ole Fejerskov
Apr 2, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·M NodaH Sano
Sep 27, 2005·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Fu-Mei HuangYu-Chao Chang
Oct 4, 2005·Dental Materials : Official Publication of the Academy of Dental Materials·J VolkW Geurtsen
Jan 24, 2006·Dental Materials : Official Publication of the Academy of Dental Materials·Keyvan MoharamzadehAndy M Scutt
Sep 26, 2006·Journal of Dental Research·H SchweiklG Schmalz
Oct 17, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yi-Ching LiYu-Chao Chang
Jul 6, 2007·Archives of Toxicology·Franz-Xaver ReichlReinhard Hickel
Jul 28, 2007·Dental Materials : Official Publication of the Academy of Dental Materials·M DemirciH Schweikl
Sep 22, 2009·Dental Materials : Official Publication of the Academy of Dental Materials·Ebru UrcanFranz Xaver Reichl
Dec 22, 2009·Biomaterials·Giuseppina NoccaGianrico Spagnuolo

❮ Previous
Next ❯

Citations

Dec 5, 2012·Dental Materials Journal·Ayse AtayPelin Guneri
Apr 17, 2015·TheScientificWorldJournal·Ivana Márcia Alves DinizMárcia Martins Marques
Oct 4, 2013·Dental Materials Journal·Kyunghwan KimHyeong-Cheol Yang
Dec 17, 2016·Brazilian Dental Journal·Fabiano Palmeira GonçalvesMíriam Zaccaro Scelza
Apr 2, 2015·Brazilian Dental Journal·Silvia T EliasAna Paula Dias Ribeiro
Jul 4, 2015·The Open Dentistry Journal·Gilvanely Cardoso Alves, Ana Paula Veras Sobral
May 21, 2019·Dental Materials Journal·Thomas R SchneiderDoug Lobner
Jun 2, 2018·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Ayşe AtayEbru Cal
Jun 2, 2018·Scanning·Gustavo Fernandes de SouzaBoniek Castillo Dutra Borges
Oct 10, 2019·Brazilian Dental Journal·Laura Alves BastosFrancisco Wanderley Garcia Paula-Silva
Jan 23, 2020·Materials·Luisa FröbSusanne Gerhardt-Szép
Jun 4, 2020·International Journal of Molecular Sciences·Adam WawrzynkiewiczIreneusz Majsterek
Jan 19, 2021·International Endodontic Journal·Y J QiuT Saito

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Dental Materials : Official Publication of the Academy of Dental Materials
Giuseppina NoccaAlessandro Lupi
Dental Materials : Official Publication of the Academy of Dental Materials
Jongryul KimDavid H Pashley
Dental Materials : Official Publication of the Academy of Dental Materials
Lorenzo BreschiDavid H Pashley
© 2021 Meta ULC. All rights reserved