Cytotoxicity of food preservatives in cultured rat hepatocytes loaded with linolenic acid

Toxicology
N SugiharaK Furuno

Abstract

We investigated the ability of eight food preservatives to induce lipid peroxidation in normal and alpha-linolenic acid (LNA)-loaded cultured rat hepatocytes. On the addition of sodium dehydroacetate (DHA-Na), potassium sorbate (SA-K) or thiabendazole (TBZ) to the cell culture, lipid peroxidation, assessed in terms of the production of malondialdehyde (MDA), was induced in LNA-loaded cells, but not in normal cells. At the low concentrations, induction of lipid peroxidation in LNA-loaded cells was highest with TBZ, whereas at high concentrations DHA-Na greatly induced lipid peroxidation. The occurrence of lipid peroxidation in LNA-loaded cells was accompanied by a decrease in cellular GSH levels with the three preservatives and by a decrease in cellular protein-SH levels with DHA-Na and TBZ. Furthermore, cell injury, measured by the release of LDH, was produced in LNA-loaded cells exposed to DHA-Na and SA-K. The addition of TBZ caused substantial cell injury in normal cells, and even greater injury in LNA-loaded cells. The prevention of lipid peroxidation in LNA-loaded hepatocytes by addition of an antioxidant, N,N'-diphenyl-p-phenylenediamine (DPPD) almost completely prevented DHA-Na- and SA-K-induced cell injury, and reduced T...Continue Reading

References

May 1, 1978·Analytical Biochemistry·M Mihara, M Uchiyama
Dec 1, 1976·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·P WiebkinJ W Bridges
Aug 1, 1992·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·R Dargel
Jan 22, 1992·Biochemical Pharmacology·Y NakagawaG A Moore
Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Aug 1, 1988·Australian and New Zealand Journal of Medicine·R A Gibson
Apr 1, 1988·The Journal of Nutrition·K L Fritsche, P V Johnston
Aug 15, 1984·The Biochemical Journal·T F Slater
Jan 1, 1983·Chemico-biological Interactions·R H ReitzP G Watanabe
May 1, 1994·Toxicology and Applied Pharmacology·N SugiharaK Kohashi
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

❮ Previous
Next ❯

Citations

Sep 5, 2002·Cancer·Hanifa BouzourenePhilippe Coucke
Jan 5, 2010·Chemical & Pharmaceutical Bulletin·Shizuyo HoriyamaMitsuo Takayama
Jul 27, 2004·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Yukio TanakaShinjiro Hori
Jan 10, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Marilyne SéïdeDiana A Averill-Bates
Aug 21, 2010·Journal of Food Science·Yansheng WangWenqing Zhang
Mar 11, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sifa Türkoğlu
Dec 19, 2002·Critical Reviews in Toxicology·E M BomhardM Richter
Apr 21, 2001·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S OzawaY Ohno
Dec 22, 2005·Journal of Agricultural and Food Chemistry·M Teresa Pérez-PriorJulio Casado

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
T KelerA Nowotny
Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
Yukio TanakaYutaka Takagaki
Food Additives and Contaminants
C FerrandG de Saint Blanquat
Journal of Agricultural and Food Chemistry
C FerrandG de Saint Blanquat
© 2021 Meta ULC. All rights reserved