Cytotoxicity of monomers, plasticizer and degradation by-products released from dental hard chairside reline resins

Dental Materials : Official Publication of the Academy of Dental Materials
Carolina de Andrade Lima ChavesCarlos Eduardo Vergani

Abstract

The aim of this study was to evaluate the cytotoxic effect of the monomers isobutyl methacrylate (IBMA) and 1,6-hexanediol dimethacrylate (1,6-HDMA), the plasticizer di-n-butyl phthalate (DBP), and the degradation by-products methacrylic acid (MA) and benzoic acid (BA) on L929 cells. Based on previous investigations on the release of these compounds from hard chairside reline resins, a range of concentrations (micromol/L) were selected for the cytotoxicity tests (IBMA, 5.49-1406.57; 1,6-HDMA, 1.22-39.32; DBP, 1.12-143.8; MA, 9.07-581; BA, 3.19-409). Cytotoxic effects were assessed using MTT and (3)H-thymidine assays after the cells had been exposed to the test compounds at the given concentrations for 24 h. Cytotoxicity was rated based on cell viability relative to controls (cells exposed to medium without test substances). DNA synthesis activity was inhibited by all compounds. Mitochondrial dehydrogenase activity decreased in cells treated with monomers, plasticizer and MA by-product, whereas no cytotoxic effect was observed on contact with BA at the majority of concentrations tested. The ranges of suppression for (3)H-thymidine assay were: IBMA, 25-95%; 1,6-HDMA, 95-98%; DBP, 40-98%; MA, 97-99%; BA, 54-71%. For MTT assay, the...Continue Reading

Citations

Nov 11, 2011·Journal of Materials Science. Materials in Medicine·Rasika P IlleperumaJin Kim
Dec 5, 2012·Dental Materials Journal·Ayse AtayPelin Guneri
Oct 9, 2014·BioMed Research International·Carolina de Andrade Lima ChavesAna Lucia Machado
Sep 17, 2011·Dental Materials : Official Publication of the Academy of Dental Materials·Canan BuralGülsen Bayraktar
May 23, 2013·Acta Odontologica Scandinavica·Jae-Sung KwonKyoung-Nam Kim
Aug 24, 2013·BioMed Research International·Cristina B NevesAna F Bettencourt
Nov 11, 2014·Journal of Tissue Engineering·Carolina Al ChavesAna L Machado
Apr 2, 2015·Brazilian Dental Journal·Silvia T EliasAna Paula Dias Ribeiro
Jul 24, 2018·The International Journal of Artificial Organs·Merve Çakırbay TanışHandan Sevim
Apr 4, 2019·TheScientificWorldJournal·Simone Kreve, Andréa C Dos Reis
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Konstantina RoussouElisabeth A Koulaouzidou

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