PMID: 20650090Jun 1, 1995Paper

Cytotoxicity of retinoic acid, menadione and aflatoxin B(1) in rat liver slices using Netwell inserts as a new culture system

Toxicology in Vitro : an International Journal Published in Association with BIBRA
W R LeemanA A Rutten

Abstract

Precision-cut rat liver slices were used to develop a new dynamic incubation system in which histomorphology and measurement of the release of lactate dehydrogenase (LDH) and the conversion of MTT were applied to evaluate cytotoxicity. Liver slices, precision-cut using a Krumdieck tissue slicer, were cultured in a new system using 200-mum polyester mesh Netwell inserts in six-well cell-culture clusters on a rocker platform at 37 degrees C and 40% O(2). The major advantage of this new culture system is the easy way in which slices can be manipulated and the culture medium be sampled or changed. Rat liver slices were exposed for 4 hr to retinoic acid (RA), menadione or aflatoxin B(1) (AFB(1)). Directly after treatment and after an additional 20-hr recovery period, histomorphological observations of slices were made, and LDH release and MTT conversion were measured. Slices exposed to RA showed dose-related cytotoxicity in the MTT assay only. The cytotoxic response to AFB(1) was more pronounced in the assay of LDH release than in the MTT assay. Histomorphology, LDH release and the MTT assay revealed cytotoxic effects induced by menadione. We conclude that culturing liver slices using Netwell inserts is a good alternative to other c...Continue Reading

References

Jan 1, 1992·Toxicology·S Azri-MeehanK Brendel
Jan 1, 1992·Toxicology and Applied Pharmacology·S AzriK Brendel
Nov 1, 1983·In Vitro·A HartJ B Balinsky
Jan 1, 1983·Molecular and Cellular Biochemistry·C Guguen-Guillouzo, A Guillouzo
May 1, 1980·Analytical Biochemistry·C L KrumdieckK J Ho
May 1, 1993·Free Radical Biology & Medicine·H ChenR C Boyle
Feb 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J A BeamandB G Lake
May 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B G LakeR J Price
Sep 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M C Wright, A J Paine

❮ Previous
Next ❯

Citations

Nov 25, 2000·Toxicology·C Lerche-Langrand, H J Toutain
Oct 18, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·W J MaasJ J van de Sandt
Jan 23, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I A M de Graaf, H J Koster
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M BlackmoreT J Gray
Jan 10, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P OlingaG M Groothuis
Dec 24, 1997·Journal of Pharmacological and Toxicological Methods·P OlingaG M Groothuis
Aug 22, 2007·Tissue Engineering·Yuet Mei KhongHanry Yu
Apr 21, 2004·Journal of Veterinary Pharmacology and Therapeutics·S SivapathasundaramC Ioannides
Dec 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Tzuen Yih SawMary Mah Lee Ng
Mar 16, 2013·Toxicology Research·Valerie Y SoldatowIvan Rusyn
Jun 19, 2021·STAR Protocols·Heidi L KenersonRaymond S Yeung

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.