PMID: 9638517Jan 1, 1997Paper

Cytotoxicity of some oxysterols on human vascular smooth muscle cells was mediated by apoptosis

Journal of Atherosclerosis and Thrombosis
Y MiyashitaH Tomioka

Abstract

A decrease in smooth muscle cells is observed in advanced atherosclerotic lesion. To understand this mechanism, we selected oxysterols as candidates for toxic lipid, and examined their cytotoxicity on human cultured vascular smooth muscle cells, together with the manner of cell death. In the presence of 7-ketocholesterol or 7 beta-hydroxycholesterol (50 mumol/L), the percentage of detached cells increased significantly with dose dependency, and an increase in detached cell number and DNA nick detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling study (TUNEL) preceded an increase in lactate dehydrogenase released into the medium. DNA extracted from smooth muscle cells incubated with 7-ketocholesterol or 7 beta-hydroxycholesterol showed a laddering pattern on agarose electrophoresis. In the presence of 7-ketocholesterol or 7 beta-hydroxycholesterol, fragmented DNA quantified by the quantitative sandwich enzyme immunoassay was significantly increased. From these results, it is proposed that 7-ketocholesterol and 7 beta-hydroxycholesterol are toxic to smooth muscle cells, and that this cytotoxicity is mediated by apoptosis.

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Citations

Feb 22, 2001·Biochemical and Biophysical Research Communications·J ChenJ M Miano
Apr 21, 2006·Diabetes Research and Clinical Practice·Tomokazu OyamaKohji Shirai
Dec 6, 2005·Metabolism: Clinical and Experimental·Junko AdachiVictor R Preedy
Feb 22, 2005·Redox Report : Communications in Free Radical Research·Makoto ShimozawaToshikazu Yoshikawa
Aug 13, 2009·Journal of Atherosclerosis and Thrombosis·Takashi HitsumotoKohji Shirai
Mar 9, 2002·Molecular and Cellular Biology·Sudesh AgrawalGuy M Chisolm
Jun 9, 2005·Journal of Atherosclerosis and Thrombosis·Yoh MiyashitaKohji Shirai
Dec 6, 2006·Journal of Atherosclerosis and Thrombosis·Masaki OhtsukaKohji Shirai
Mar 23, 2018·Journal of Endocrinological Investigation·A SamadiI Lay
Sep 20, 2002·Journal of Atherosclerosis and Thrombosis·Yoh MiyashitaKohji Shirai
Sep 19, 2002·Journal of Atherosclerosis and Thrombosis·Tomokazu OyamaTakeshi Yagima
Nov 27, 2002·European Journal of Pharmacology·Manabu UemuraToshikazu Yoshikawa

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