Cytotoxicity of the Bacillus cereus Nhe enterotoxin requires specific binding order of its three exoprotein components.

Infection and Immunity
Toril LindbäckErwin Märtlbauer

Abstract

This study focuses on the interaction of the three components of the Bacillus cereus Nhe enterotoxin with particular emphasis on the functional roles of NheB and NheC. The results demonstrated that both NheB and NheC were able to bind to Vero cells directly while NheA lacked this ability. It was also shown that Nhe-induced cytotoxicity required a specific binding order of the individual components whereby the presence of NheC in the priming step as well as the presence of NheA in the final incubation step was mandatory. Priming of cells with NheB alone and addition of NheA plus NheC in the second step failed to induce toxic effects. Furthermore, in solution, excess NheC inhibited binding of NheB to Vero cells, whereas priming of cells with excess NheC resulted in full toxicity if unbound NheC was removed before addition of NheB. By using mutated NheC proteins where the two cysteine residues in the predicted beta-tongue were replaced with glycine (NheCcys-) or where the entire hydrophobic stretch was deleted (NheChr-), the predicted hydrophobic beta-tongue of NheC was found essential for binding to cell membranes but not for interaction with NheB in solution. All data presented here are compatible with the following model. The f...Continue Reading

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Citations

Sep 17, 2015·Biochimica Et Biophysica Acta·Katia CosentinoAna J García-Sáez
Nov 12, 2013·Toxicon : Official Journal of the International Society on Toxinology·Nadja JeßbergerErwin Märtlbauer
Jun 1, 2016·Frontiers in Microbiology·Kui ZhuShuangyang Ding
Oct 21, 2016·Cellular Microbiology·Xiaoye LiuKui Zhu
Nov 28, 2013·Chemical Communications : Chem Comm·Kui ZhuErwin Märtlbauer
Mar 23, 2011·Critical Reviews in Microbiology·Siele CeuppensMieke Uyttendaele
Sep 6, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Danh PhungPeter J Artymiuk
Aug 26, 2018·Toxins·Bastian Bräuning, Michael Groll
Feb 8, 2020·Nature Communications·Daniel FoxSi Ming Man
Nov 11, 2020·Toxins·Nadja JessbergerErwin Märtlbauer
Aug 20, 2019·Food Microbiology·Nadja JessbergerErwin Märtlbauer
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Andreja RajkovicMieke Uyttendaele
Feb 3, 2021·Toxins·Richard DietrichPer Einar Granum
Oct 3, 2020·Trends in Microbiology·Daniel Enosi TuipulotuSi Ming Man
Jun 24, 2021·Comprehensive Reviews in Food Science and Food Safety·Jelena JovanovicAndreja Rajkovic
Jul 31, 2021·Frontiers in Microbiology·Paul Jakob SchmidClemens Kittinger

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