Cytotoxicity on human cells of Cry1Ab and Cry1Ac Bt insecticidal toxins alone or with a glyphosate-based herbicide

Journal of Applied Toxicology : JAT
Robin MesnageG-E Séralini

Abstract

The study of combined effects of pesticides represents a challenge for toxicology. In the case of the new growing generation of genetically modified (GM) plants with stacked traits, glyphosate-based herbicides (like Roundup) residues are present in the Roundup-tolerant edible plants (especially corns) and mixed with modified Bt insecticidal toxins that are produced by the GM plants themselves. The potential side effects of these combined pesticides on human cells are investigated in this work. Here we have tested for the very first time Cry1Ab and Cry1Ac Bt toxins (10 ppb to 100 ppm) on the human embryonic kidney cell line 293, as well as their combined actions with Roundup, within 24 h, on three biomarkers of cell death: measurements of mitochondrial succinate dehydrogenase, adenylate kinase release by membrane alterations and caspase 3/7 inductions. Cry1Ab caused cell death from 100 ppm. For Cry1Ac, under such conditions, no effects were detected. The Roundup tested alone from 1 to 20 000 ppm is necrotic and apoptotic from 50 ppm, far below agricultural dilutions (50% lethal concentration 57.5 ppm). The only measured significant combined effect was that Cry1Ab and Cry1Ac reduced caspases 3/7 activations induced by Roundup; th...Continue Reading

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Citations

Aug 29, 2014·BioMed Research International·Davi Felipe FariasAna Fontenele Urano Carvalho
Jun 25, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jie MaoQing Xia
Jul 11, 2016·The International Journal of Biochemistry & Cell Biology·Marilu Torres-MartínezLeticia Moreno-Fierros
Dec 27, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jessica NardiLuciana Grazziotin Rossato-Grando
Sep 20, 2018·Risk Analysis : an Official Publication of the Society for Risk Analysis·Rani Lill Anjum, Elena Rocca
Aug 16, 2019·Journal of the Science of Food and Agriculture·Karuna RupulaSashidhar Rao Beedu
Nov 6, 2015·Journal of Applied Toxicology : JAT·Néstor Rubio-Infante, Leticia Moreno-Fierros
Jun 14, 2019·Frontiers in Bioengineering and Biotechnology·Siguna Mueller
Aug 5, 2019·Current Pharmaceutical Biotechnology·Gretel Mendoza-AlmanzaJorge Olmos
Jan 9, 2021·In Vitro Cellular & Developmental Biology. Animal·I Bautista-JacoboL Moreno-Fierros
Jul 29, 2021·Journal of Agricultural and Food Chemistry·Aroosha FaheemYonggang Hu

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