Cytotoxicity study of high temperature wool (HT wool) by cell magnetometric evaluation

Journal of Occupational Health
Yuichiro KudoYoshiharu Aizawa

Abstract

We performed a cytotoxicity study by cell magnetometry, measured lactate dehydrogenase (LDH) activity by enzyme assay, detected DNA ladder formation, and performed morphological examination by electron microscopy in order to evaluate the safety of high temperature wool (HT wool), an asbestos substitute, using long and short chrysotile fibers (CF) as positive controls and phosphate buffered saline (PBS) as a negative control. Alveolar macrophages were isolated from male Fisher rats. Following the addition of iron oxide particles (Fe(3)O(4)) to macrophages, HT wool, long or short CF was added. Then, the remanence strength was measured for 20 min after magnetization by an external field. Percent LDH release was calculated after determining LDH activity. DNA was detected using an apoptosis detection kit. Morphological observation was performed by taking electron micrographs of macrophages in the groups treated with HT wool and long- and short-CF. Rapid relaxation, an indicator of decay of cytotoxicity, was observed by cell magnetometry immediately after magnetization was ended in the groups treated with HT wool and PBS, showing that HT wool causes no harmful effect on the cytoskeleton. The CF-treated groups had higher LDH activity ...Continue Reading

References

Feb 1, 1990·The American Review of Respiratory Disease·Y Sibille, H Y Reynolds
May 1, 1988·British Journal of Industrial Medicine·J M DavisT Smith
Jan 1, 1984·Journal of Toxicology and Environmental Health·W H PailesV Castranova
Sep 1, 1983·Environmental Health Perspectives·I P GormleyA Wright
May 1, 1981·British Journal of Industrial Medicine·G Berry
Oct 1, 1995·Physiological Reviews·R B Maccioni, V Cambiazo
Jan 1, 1993·Cell Motility and the Cytoskeleton·L Cassimeris
Nov 1, 1996·Toxicology Letters·W MöllerJ Heyder
Nov 1, 1996·The American Journal of Physiology·R F HamiltonA Holian
Nov 1, 1996·Biulleten' eksperimental'noĭ biologii i meditsiny·G M Kharin, A Z Shakirova
Mar 1, 1997·Journal of Structural Biology·W Brinkley
Jul 31, 1998·The Annals of Occupational Hygiene·O KamstrupM Guldberg
May 1, 1999·The Tohoku Journal of Experimental Medicine·T KeiraM Kotani
Jan 3, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·B S WinderM G Miller
Jul 14, 2001·Inhalation Toxicology·O KamstrupP Thévenaz
Aug 9, 2002·Journal of Toxicology and Environmental Health. Part a·Mitsuyasu WatanabeMakoto Kotani
Jan 30, 2003·Journal of Toxicology and Environmental Health. Part a·Masato NiitsuyaMakato Kotani
Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·F WROBLEWSKI, J S LADUE

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

The Journal of Toxicological Sciences
Yuichiro KudoYoshiharu Aizawa
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
Jian-Jun DengYa-Li Zeng
© 2021 Meta ULC. All rights reserved