PMID: 9177243Jun 10, 1997Paper

D-Ala-D-Ala ligases from glycopeptide antibiotic-producing organisms are highly homologous to the enterococcal vancomycin-resistance ligases VanA and VanB

Proceedings of the National Academy of Sciences of the United States of America
C G MarshallGerard D Wright

Abstract

The crisis in antibiotic resistance has resulted in an increasing fear of the emergence of untreatable organisms. Resistance to the glycopeptide antibiotic vancomycin in the enterococci, and the spread of these pathogens throughout the environment, has shown that this scenario is a matter of fact rather than fiction. The basis for vancomycin resistance is the manufacture of the depsipeptide D-Ala-D-lactate, which is incorporated into the peptidoglycan cell wall in place of the vancomycin target D-Ala-D-Ala. Pivotal to the resistance mechanism is the production of a D-Ala-D-Ala ligase capable of ester formation. Two highly efficient depsipeptide ligases have been cloned from vancomycin-resistant enterococci: VanA and VanB. These ligases show high amino acid sequence similarity to each other ( approximately 75%), but less so to other D-Ala-D-X ligases (<30%). We have cloned ddls from two glycopeptide-producing organisms, the vancomycin producer Amycolatopsis orientalis and the A47934 producer Streptomyces toyocaensis. These ligases show strong predicted amino acid homology to VanA and VanB (>60%) but not to other D-Ala-D-X ligases (<35%). The D-Ala-D-Ala ligase from S. toyocaensis shows D-Ala-D-lactate synthase activity in cell-f...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1990·Clinical Microbiology Reviews·B E Murray
Apr 1, 1989·Computer Applications in the Biosciences : CABIOS·D G Higgins, P M Sharp
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R Benveniste, J Davies
Oct 1, 1994·The Journal of Antimicrobial Chemotherapy·J BatesD T Griffiths
Mar 1, 1994·The Journal of Antimicrobial Chemotherapy·C TorresF Baquero
Aug 1, 1993·Antimicrobial Agents and Chemotherapy·M Arthur, P Courvalin
Nov 1, 1993·Antimicrobial Agents and Chemotherapy·V Webb, J Davies
Apr 13, 1996·Lancet·F Howarth, D Poulter
Apr 13, 1996·Lancet·M EggerG Antes
May 18, 1996·Lancet·A Mudd
Jun 29, 1996·Lancet·R Wise
Jun 29, 1996·Lancet·A PlyusninA Vaheri
Nov 1, 1996·Antimicrobial Agents and Chemotherapy·T M CoqueB E Murray

❮ Previous
Next ❯

Citations

May 7, 2010·Journal of Industrial Microbiology & Biotechnology·Eric Cundliffe, Arnold L Demain
Jan 4, 2001·International Journal of Antimicrobial Agents·Y Gholizadeh, P Courvalin
Jul 24, 2002·International Journal of Antimicrobial Agents·F M E WagenlehnerK G Naber
Oct 6, 1999·Current Opinion in Microbiology·D A Rowe-Magnus, D Mazel
Apr 13, 2010·Nature Chemical Biology·Kalinka KotevaGerard D Wright
Apr 21, 2010·Nature Chemical Biology·Michel Arthur
Feb 6, 2007·Nature Reviews. Microbiology·Gerard D Wright
Jan 8, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David M Livermore
Mar 24, 2010·Antimicrobial Agents and Chemotherapy·Giorgia Letizia MarconeFlavia Marinelli
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Jeff PootoolalGerard D Wright
Jun 18, 2004·Emerging Infectious Diseases·Karen LuJulian Davies
Mar 2, 2002·The Lancet Infectious Diseases·M J BontenR A Weinstein
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·I A Lessard, C T Walsh
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jeff PootoolalGerard D Wright
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Peter SpanogiannopoulosGerard D Wright
Jan 31, 2003·Expert Opinion on Investigational Drugs·Neil Woodford
Aug 1, 2010·Expert Opinion on Drug Discovery·Gerard D Wright
Jun 7, 2005·Expert Opinion on Emerging Drugs·Florian M E WagenlehnerKurt G Naber
Apr 7, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·N Woodford
Oct 24, 2007·Current Opinion in Microbiology·Vanessa M D'CostaGerard D Wright
Apr 2, 2003·Journal of Veterinary Pharmacology and Therapeutics·B CatryA De Kruif
Apr 10, 2008·Journal of Internal Medicine·P Courvalin
Sep 16, 2015·BMC Evolutionary Biology·Daniel C Schlatter, Linda L Kinkel
Feb 24, 2006·Chemistry & Biology·Sherry S LambGerard D Wright
Aug 11, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Teresa M. Barbosa, Stuart B. Levy
Dec 21, 2017·The ISME Journal·Andrew C PawlowskiGerard D Wright
Jan 7, 2015·Nature·Losee L LingKim Lewis
Jan 8, 2014·Antimicrobial Agents and Chemotherapy·Jean-Emmanuel HugonnetMichel Arthur
Jun 9, 2016·Antimicrobial Agents and Chemotherapy·Lizah T van der AartGilles P van Wezel
Apr 23, 2016·Nature Communications·Peter J StogiosGerard D Wright
Jan 1, 2014·Antibiotics·Elisa BindaGiorgia Letizia Marcone
Jul 14, 2020·Frontiers in Microbiology·Oleksandr YushchukFlavia Marinelli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.