PMID: 238Jul 1, 1975

D-alanyl-D-alanine carboxypeptidase in the bacterial form and L-form of Proteus mirabilis

European Journal of Biochemistry
H H MartinR Burger

Abstract

Membranes of the bacterial form and the stable and unstable L-forms of Proteus mirabilis contain LD and DD-carboxypeptidase. The DD-carboxypeptidase is inhibited non-competitively by penicillin G. The enzyme of the bacterial form is highly penicillin-sensitive (Ki - 4 X 10(-9) M penicillin G). Inhibition is only partly reversible by treatment with penicillinase or by dialysis against buffer. In contrast, the DD-carboxypeptidase of the unstable L-form, grown in the presence of penicillin, is 175-fold less penicillin-sensitive (Ki = 7 X 10(7) M penicillin G). Inhibition is completely reversed by penicillinase or dialysis. After inhibition by penicillin and subsequent reactivation the penicillin sensitivity of the bacterial DD-carboxtpeptidase is similar to the sensitivity of the enzyme of the unstable L-form. The hypothesis is proposed that P. mirabilis contains two DD-carboxypeptidases of different penicillin sensitivity and with different mechanisms of penicillin binding. Peptidoglycan synthesis in the cell walls of the unstable L-form is probably carried out with the help of only one DD-carboxypeptidase, viz. the completely reactivatable enzyme with the lower penicillin sensitivity.

References

Apr 8, 1974·Biochemical and Biophysical Research Communications·B OppenheimA Patchornik
May 15, 1974·European Journal of Biochemistry·J CoyetteJ M Ghuysen
May 10, 1974·Annals of the New York Academy of Sciences·P E Reynolds, H J Barnett
Aug 13, 1968·Biochemical and Biophysical Research Communications·E MuñozM R Salton
May 22, 1970·Biochemical and Biophysical Research Communications·W Katz, H H Martin
Dec 31, 1971·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J L StromingerG G Wickus
May 10, 1974·Annals of the New York Academy of Sciences·J M GhuysenA Marquet
Feb 1, 1974·European Journal of Biochemistry·M Nguyen-DistècheM R Salton
Jan 1, 1973·Biochimie·B GondréJ Van Heijenoort
Jan 1, 1973·The Biochemical Journal·M NietoJ M Ghuysen
Apr 1, 1968·Journal of General Microbiology·P H Hofschneider, H H Martin
May 14, 1969·Journal of Molecular Biology·U SchwarzH Frank
May 25, 1971·Biochemistry·M Leyh-BouilleM Nieto
Mar 28, 1972·Biochemistry·M Leyh-BouilleH R Perkins
Jun 24, 1967·Nature·A N ChatterjeeH R Perkins

Citations

Oct 1, 1980·Archives of Microbiology·H P KrollH H Martin
Sep 1, 1977·European Journal of Biochemistry·S T ShepherdP E Reynolds
Aug 1, 1982·European Journal of Biochemistry·A Rodríguez-TébarD Vázquez
Nov 15, 1983·European Journal of Biochemistry·J A ToddD J Ellar
Jan 1, 1988·Biochemical Pharmacology·J M FrèreM Crine
Apr 7, 1983·Journal of Theoretical Biology·J M FrèreA Renard
Apr 1, 1979·European Journal of Biochemistry·H H Martin, J Gmeiner
Jan 1, 1985·Critical Reviews in Microbiology·J M Frère, B Joris
Apr 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kannan Rajavelu, Perumal Rajakumar

Related Concepts

Abufne
Carboxypeptidase
Plasma Membrane
D-Amino Acid Dehydrogenase
Synthetic Detergent
Hydrogen-Ion Concentration
L Forms
Van-Pen-G
Vegacillin
Proteus mirabilis

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