D-Dimer and Fibrin Degradation Products Impair Platelet Signaling: Plasma D-Dimer Is a Predictor and Mediator of Platelet Dysfunction During Trauma.

The Journal of Applied Laboratory Medicine
C C VerniS L Diamond

Abstract

Platelet dysfunction often accompanies trauma-induced coagulopathy. Because soluble fibrin impairs platelet glycoprotein VI (GPVI) signaling and platelets of trauma patients can display impaired calcium mobilization, we explored the role of fibrinolysis on platelet dysfunction during trauma. Convulxin-induced GPVI calcium mobilization was investigated in healthy platelet-rich plasma (PRP) pretreated with thrombin and tissue plasminogen activator (tPA). Blood samples from healthy participants (n = 7) and trauma patients (n = 22) were tested for platelet calcium mobilization, plasma D-dimer, platelet D-dimer binding (via flow cytometry), and platelet lumi-aggregometry. For healthy platelets, maximal platelet dysfunction was observed when cross-linked soluble fibrin (no tPA) or cross-linked fibrin degradation products (FDPs) were generated in suspension before convulxin stimulation. Lack of fibrin polymerization (inhibited by Gly-Pro-Arg-Pro [GPRP]) or lack of factor XIIIa cross-linking (T101-inhibited) restored GPVI signaling, whereas non-cross-linked FDPs only partially blocked signaling induced by convulxin. In addition, D-dimer added to healthy PRP impaired platelet aggregation and dense granule release induced by various agon...Continue Reading

References

Aug 1, 1977·The American Journal of Physiology·K G Orloff, D Michaeli
Aug 1, 1980·The American Journal of Medicine·F I ParetiP M Mannucci
Apr 1, 1994·Journal of Neurosurgical Anesthesiology·S Bredbacka, G Edner
Sep 26, 2003·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Hideo WadaTsutomu Nobori
Sep 9, 2004·Seminars in Thrombosis and Hemostasis·Peter N Walsh
Oct 14, 2008·The Journal of Trauma·John R HessBertil Bouillon
Aug 18, 2009·Transfusion·Nancy M Dunbar, Wayne L Chandler
Jun 22, 2010·Nature Biotechnology·Manash S ChatterjeeScott L Diamond
Apr 24, 2012·Journal of the American College of Surgeons·Max V WohlauerMark Walsh
Jun 30, 2012·The Journal of Trauma and Acute Care Surgery·Matthew E KutcherMitchell Jay Cohen
Feb 20, 2016·The Journal of Trauma and Acute Care Surgery·Matthew T RamseyLisa K Jennings
Jul 7, 2016·Blood·Ronald ChangJohn B Holcomb
Aug 18, 2016·European Heart Journal. Cardiovascular Pharmacotherapy·Armaghan Y SoomroGeorge D Dangas
Apr 30, 2017·The Journal of Trauma and Acute Care Surgery·Paul VulliamyRoss A Davenport
Aug 15, 2017·Methods in Molecular Biology·Anne-Mette Hvas, Emmanuel J Favaloro
Oct 6, 2017·Journal of Thrombosis and Haemostasis : JTH·M Y LeeS L Diamond
Jan 4, 2018·Blood Advances·Marie-Blanche OnselaerSteve P Watson
Aug 16, 2018·Platelets·Alexandre SlaterSteve P Watson
Oct 13, 2018·Thrombosis and Haemostasis·Majed A RefaaiPhoenix D Bell
Dec 12, 2018·The Journal of Trauma and Acute Care Surgery·Christopher C VerniScott L Diamond
Apr 16, 2019·Journal of Thrombosis and Haemostasis : JTH·Lucy Z KornblithMitchell J Cohen

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Citations

Sep 22, 2021·Archives of Orthopaedic and Trauma Surgery·Anna Jungwirth-WeinbergerFriedrich Boettner

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