D-Galactose induces cellulase gene expression in Hypocrea jecorina at low growth rates

Microbiology
Levente KaraffaBernhard Seiboth

Abstract

Lactose (1,4-O-beta-d-galactopyranosyl-d-glucose) is a soluble and economic carbon source for the industrial production of cellulases or recombinant proteins by Hypocrea jecorina (anamorph Trichoderma reesei). The mechanism by which lactose induces cellulase formation is not understood. Recent data showed that the galactokinase step is essential for cellulase induction by lactose, but growth on d-galactose alone does not induce cellulases. Consequently, the hypothesis was tested that d-galactose may be an inducer only at a low growth rate, which is typically observed when growing on lactose. Carbon-limited chemostat cultivations of H. jecorina were therefore performed at different dilution rates with d-galactose, lactose, galactitol and d-glucose. Cellulase gene expression was monitored by using a strain carrying a fusion between the cbh2 (encoding cellobiohydrolase 2, Cel6A) promoter region and the Aspergillus niger glucose oxidase gene and by identification of the two major cellobiohydrolases Cel7A and Cel6A. The results show that d-galactose indeed induces cbh2 gene transcription and leads to Cel7A and Cel6A accumulation at a low (D=0.015 h(-1)) but not at higher dilution rates. At the same dilution rate, growth on d-glucose...Continue Reading

References

Sep 1, 1979·Journal of Bacteriology·D Sternberg, G R Mandels
Jan 1, 1983·Methods in Enzymology·G L Peterson
Aug 19, 2003·The Journal of Biological Chemistry·Hazel M HoldenJames B Thoden
Nov 19, 2003·Archives of Microbiology·Erzsébet FeketeChristian P Kubicek
May 7, 2004·European Journal of Biochemistry·Manuela PailChristian P Kubicek
Feb 5, 2005·Applied and Environmental Microbiology·Bernhard SeibothChristian P Kubicek
Mar 30, 2005·The Journal of Biological Chemistry·James B Thoden, Hazel M Holden
Aug 17, 2005·FEMS Microbiology Reviews·Nina AroMerja Penttilä

❮ Previous
Next ❯

Citations

Jan 10, 2013·Applied Microbiology and Biotechnology·Levente KaraffaChristian P Kubicek
May 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erzsébet FeketeLevente Karaffa
Aug 9, 2011·Applied and Environmental Microbiology·Ulla ChristensenRonald P de Vries
Sep 3, 2009·Biotechnology for Biofuels·Christian P KubicekBernhard Seiboth
Feb 22, 2014·PloS One·Maria Augusta Crivelente HortaAnete Pereira Souza
Apr 12, 2014·Applied Microbiology and Biotechnology·Shuji TaniTetsuo Kobayashi
Sep 23, 2008·Journal of Bioscience and Bioengineering·Xu FangShigeki Sawayama
Aug 20, 2015·Applied Biochemistry and Biotechnology·Yagya Prasad Paudel, Wensheng Qin
Feb 14, 2012·FEMS Microbiology Letters·Erzsébet FeketeLevente Karaffa
Jun 16, 2017·Biotechnology for Biofuels·Tiziano BenocciRonald P de Vries
Nov 27, 2018·Biotechnology for Biofuels·Gen ZouZhihua Zhou
Feb 28, 2019·Journal of Industrial Microbiology & Biotechnology·Nayani Dhanushka DaranagamaWataru Ogasawara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.