D-galactosyl-beta1-1'-sphingosine and D-glucosyl-beta1-1'-sphingosine induce human natural killer cell apoptosis

Biochemical and Biophysical Research Communications
Azzam A MaghazachiFarrukh A Chaudhry

Abstract

Natural killer (NK) cells perform multiple biological functions including tumor cell lysis and eradicating virally infected cells. Here, we report for the first time that D-galactosyl-beta1-1' sphingosine and D-glucosyl-beta1- 1' sphingosine damage human NK cells. We show that these cells express T-cell-associated gene-8, the receptor for glycosphingolipids. D-galactosyl-beta1-1' sphingosine and D-glucosyl-beta1-1' sphingosine induce the in vitro chemotaxis of human NK cells. Both D-galactosyl-beta1-1' sphingosine and D-glucosyl-beta1-1' sphingosine inhibit the cytotoxicity and IFN-gamma secretion by these cells. Further analysis shows that the glycosphingolipids D-galactosyl-beta1-1' sphingosine and D-glucosyl-beta1-1' sphingosine but not any other lipid examined, which include D-lactosyl-beta1-1' sphingosine, sphingosine 1-phosphate, sphingosine, lysophosphatidic acid, and phosphatidic acid, induce the apoptosis, globoid-like formation, and multinucleation in human NK cells. These results may have important implications on diseases where glycosphingolipids accumulate.

References

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Citations

Nov 22, 2005·Acta Pharmacologica Sinica·Dong-Soon Im
Oct 14, 2010·Journal of Leukocyte Biology·Linda Monaco-ShawverJordan S Orange
Sep 28, 2014·Gene·Adriana Carol Eleonora Graziano, Venera Cardile
Dec 21, 2004·Molecular Immunology·Azzam A Maghazachi
Apr 29, 2009·Journal of Cellular and Molecular Medicine·Yassin Al-FalahiAzzam A Maghazachi
Sep 18, 2016·Journal of Neuroscience Research·Rosanna AvolaVenera Cardile
Dec 25, 2004·The Journal of Biological Chemistry·Satoshi IshiiTakao Shimizu

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