PMID: 1257Nov 15, 1975

D-glucose-6-phosphate dehydrogenase (Entner-Doudoroff enzyme) from Pseudomonas fluorescens. Purification, properties and regulation

European Journal of Biochemistry
D LessmannG Kurz

Abstract

1. The existence of two different D-glucose-6-phosphate dehydrogenases in Pseudomonas fluorescens has been demonstrated. Based on their different specificity and their different metabolic regulation one enzyme is appointed to the Entner-Doudoroff pathway and the other to the hexose monophosphate pathway. 2. A procedure is described for the isolation of that D-glucose-6-phosphate dehydrogenase which forms part of the Entner-Doudoroff pathway (Entner-Doudoroff enzyme). A 950-fold purification was achieved with an overall yield of 44%. The final preparation, having a specific activity of about 300 mumol NADH formed per min per mg protein, was shown to be homogeneous. 3. The molecular weight of the Entner-Doudoroff enzyme has been determined to be 220000 by gel permeation chromatography, and that of the other enzyme (Zwischenferment) has been shown to be 265000. 4. The pI of the Entner-Doudoroff enzyme has been shown to be 5.24 and that of the Zwischenferment 4.27. The Entner-Doudoroff enzyme is stable in the range of pH 6 to 10.5 and shows its maximal activity at pH 8.9. 5. The Entner-Doudoroff enzyme showed specificity for NAD+ as well as for NADP+ and exhibited homotropic effects for D-glucose 6-phosphate. It is inhibited by ATP...Continue Reading

References

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Citations

Dec 1, 1977·Experimental Parasitology·S FunayamaL A Veiga
Mar 7, 2006·Applied and Environmental Microbiology·Aditya BasuPrashant S Phale
Dec 25, 2015·FEBS Open Bio·K OlavarriaJosé Gregório Cabrera Gomez
Jun 1, 2006·FEMS Microbiology Letters·Aditya Basu, Prashant S Phale
Jul 6, 2007·Journal of Molecular Biology·Stan J J BrounsJohn van der Oost
Jul 1, 1986·The Journal of Experimental Zoology·J Jeffery, I Wood
Mar 22, 2014·The Journal of Biological Chemistry·Sebastian LaassDietmar Schomburg
Jul 17, 1998·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C S Tsai, Q Chen
Nov 25, 2019·Biochimica Et Biophysica Acta. Proteins and Proteomics·Edaena Benítez-RangelRoberto Velasco-García

Related Concepts

Drug Stability
Glucosephosphate Dehydrogenase
Hexosephosphates
Hydrogen-Ion Concentration
Alloenzymes
NADH
NADP
Pseudomonas fluorescens
Spectrophotometry, Ultraviolet
Molecular Stereochemistry

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