D-repeat in the XIST gene is required for X chromosome inactivation

RNA Biology
Qingyan LvLiangxue Lai

Abstract

XIST is a long non-coding RNA, which expressed exclusively from the inactive X chromosome. Although it has been revealed that the A-repeat contributes to the X chromosome inactivation (X-inactivation), the role of the longest D-repeat has not yet been investigated. Here, a sgRNA directed CRISPR/Cas9 system which have multiple target sites within repeat D of XIST, were used to generate D-repeat deletion and studied its roles on X-inactivation. The results showed that the deletion of D-repeat caused a significantly decreased expression of XIST, and up regulated expression of X-linked genes, suggesting that the D-repeat may play an important role in the regulation of XIST expression and silencing of the X-linked genes, which could provide a new idea in the molecular mechanisms of X-inactivation.

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Citations

Apr 14, 2016·Seminars in Cell & Developmental Biology·Stefan F Pinter
May 20, 2016·International Journal of Molecular Sciences·Rui LiYunbo Luo
Oct 26, 2016·Clinics and Research in Hepatology and Gastroenterology·Weijie MaYufeng Yuan
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Aug 29, 2019·EMBO Reports·Aurélie BousardSimão Teixeira da Rocha
Jan 5, 2020·Development·Catherine PatratClaire Rougeulle
Oct 3, 2020·Computational and Structural Biotechnology Journal·Yuanyuan Xu, Zhanjun Li
Mar 9, 2021·RNA Biology·Chong Tong, Yafei Yin
Mar 23, 2021·PLoS Genetics·Thomas Dixon-McDougall, Carolyn J Brown
Apr 4, 2021·International Journal of Molecular Sciences·Hanna Sas-Nowosielska, Adriana Magalska
Apr 17, 2021·Wiley Interdisciplinary Reviews. RNA·Jackson B TrotmanJ Mauro Calabrese
Apr 27, 2021·Non-coding RNA Research·Zhenxing SongChuan Huang
Nov 26, 2021·Biochemical Society Transactions·Alessandra Spaziano, Dr Irene Cantone
Dec 10, 2021·Biochemical Society Transactions·Ana Cláudia RaposoSimão Teixeira da Rocha

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Methods Mentioned

BETA
Transfection
PCR

Software Mentioned

Lucia
DNAman
Graphpad prism

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