Abstract
XIST is a long non-coding RNA, which expressed exclusively from the inactive X chromosome. Although it has been revealed that the A-repeat contributes to the X chromosome inactivation (X-inactivation), the role of the longest D-repeat has not yet been investigated. Here, a sgRNA directed CRISPR/Cas9 system which have multiple target sites within repeat D of XIST, were used to generate D-repeat deletion and studied its roles on X-inactivation. The results showed that the deletion of D-repeat caused a significantly decreased expression of XIST, and up regulated expression of X-linked genes, suggesting that the D-repeat may play an important role in the regulation of XIST expression and silencing of the X-linked genes, which could provide a new idea in the molecular mechanisms of X-inactivation.
References
Oct 30, 1992·Cell·N BrockdorffS Rastan
Oct 30, 1992·Cell·C J BrownH F Willard
Jan 3, 1991·Nature·C J BrownH F Willard
Jan 10, 2002·Nature Genetics·Anton WutzRudolf Jaenisch
May 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Inmaculada Hernández-MuñozMaarten van Lohuizen
Jun 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jennifer C ChowCarolyn J Brown
Aug 30, 2008·Annual Review of Genetics·Bernhard Payer, Jeannie T Lee
Nov 28, 2008·Development·Yuko HokiTakashi Sado
Feb 7, 2013·ELife·Martin JinekJennifer Doudna
Jun 25, 2013·Nature Biotechnology·Yanfang FuJeffry D Sander
Jul 23, 2013·Nature Biotechnology·Patrick D HsuFeng Zhang
Aug 3, 2013·Nature Biotechnology·Prashant MaliGeorge M Church
Aug 7, 2013·Epigenetics & Chromatin·Jakub MinksCarolyn J Brown
Aug 13, 2013·Nature Biotechnology·Vikram PattanayakDavid R Liu
Sep 3, 2013·Nucleic Acids Research·Wataru FujiiKunihiko Naito
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Apr 4, 2014·Genome Research·Suresh RamakrishnaHyongbum Kim
Nov 14, 2014·Plant & Cell Physiology·Masaki EndoSeiichi Toki
Apr 2, 2015·Archives of Toxicology·Andrea PellagattiJacqueline Boultwood
Citations
Apr 14, 2016·Seminars in Cell & Developmental Biology·Stefan F Pinter
May 20, 2016·International Journal of Molecular Sciences·Rui LiYunbo Luo
Oct 26, 2016·Clinics and Research in Hepatology and Gastroenterology·Weijie MaYufeng Yuan
Sep 28, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Asun Monfort, Anton Wutz
Aug 29, 2019·EMBO Reports·Aurélie BousardSimão Teixeira da Rocha
Feb 21, 2019·Nucleic Acids Research·Hyeon J LeeJin-Wu Nam
Jan 5, 2020·Development·Catherine PatratClaire Rougeulle
Oct 3, 2020·Computational and Structural Biotechnology Journal·Yuanyuan Xu, Zhanjun Li
Mar 9, 2021·RNA Biology·Chong Tong, Yafei Yin
Mar 23, 2021·PLoS Genetics·Thomas Dixon-McDougall, Carolyn J Brown
Apr 4, 2021·International Journal of Molecular Sciences·Hanna Sas-Nowosielska, Adriana Magalska
Apr 17, 2021·Wiley Interdisciplinary Reviews. RNA·Jackson B TrotmanJ Mauro Calabrese
Apr 27, 2021·Non-coding RNA Research·Zhenxing SongChuan Huang
Nov 26, 2021·Biochemical Society Transactions·Alessandra Spaziano, Dr Irene Cantone
Dec 10, 2021·Biochemical Society Transactions·Ana Cláudia RaposoSimão Teixeira da Rocha