d-Serine Degradation by Proteus mirabilis Contributes to Fitness during Single-Species and Polymicrobial Catheter-Associated Urinary Tract Infection.

MSphere
Aimee L BrauerChelsie E Armbruster

Abstract

Proteus mirabilis is a common cause of catheter-associated urinary tract infection (CAUTI) and secondary bacteremia, which are frequently polymicrobial. We previously utilized transposon insertion-site sequencing (Tn-Seq) to identify novel fitness factors for colonization of the catheterized urinary tract during single-species and polymicrobial infection, revealing numerous metabolic pathways that may contribute to P. mirabilis fitness regardless of the presence of other cocolonizing organisms. One such "core" fitness factor was d-serine utilization. In this study, we generated isogenic mutants in d-serine dehydratase (dsdA), d-serine permease (dsdX), and the divergently transcribed activator of the operon (dsdC) to characterize d-serine utilization in P. mirabilis and explore the contribution of this pathway to fitness during single-species and polymicrobial infection. P. mirabilis was capable of utilizing either d- or l-serine as a sole carbon or nitrogen source, and dsdA, dsdX, and dsdC were each specifically required for d-serine degradation. This capability was highly conserved among P. mirabilis isolates, although not universal among uropathogens: Escherichia coli and Morganella morganii utilized d-serine, while Providenc...Continue Reading

References

Mar 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R R MuderA M Goetz
Aug 1, 1988·Journal of the American Geriatrics Society·D RudmanD E Mattson
Nov 1, 1987·Journal of Clinical Microbiology·H L Mobley, J W Warren
Nov 1, 1985·Journal of Clinical Microbiology·H L MobleyJ W Warren
May 1, 1973·Journal of Bacteriology·S D Cosloy, E McFall
Dec 1, 1982·The Journal of Infectious Diseases·J W WarrenW C Anthony
Nov 1, 1995·Journal of Bacteriology·M Nørregaard-MadsenP Valentin-Hansen
Sep 1, 1994·The Journal of Hospital Infection·Y Siegman-IgraN Konforti
Jan 1, 1994·Scandinavian Journal of Infectious Diseases·C Watanakunakorn, S C Perni
Mar 26, 1998·Biological & Pharmaceutical Bulletin·Y HuangK Imai
Dec 10, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Joseph M MylotteSusan Goodnough
Apr 16, 2003·Scandinavian Journal of Infectious Diseases·Baek-Nam KimJiso Ryu
Jul 10, 2003·Infectious Disease Clinics of North America·Betsy Foxman, Patricia Brown
Aug 3, 2005·Drugs & Aging·Lindsay E Nicolle
Sep 6, 2006·Journal of Bacteriology·Andrew T Anfora, Rodney A Welch
Apr 1, 2008·Journal of Bacteriology·Melanie M PearsonHarry L T Mobley
Aug 23, 2008·Journal of Medical Microbiology·Stephanie D HimpslHarry L T Mobley
Aug 14, 2009·FEMS Microbiology Letters·Türkân SakinçSören G Gatermann
Feb 23, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thomas M HootonUNKNOWN Infectious Diseases Society of America
Feb 22, 2011·Journal of the American Medical Directors Association·Emily LubartArthur Leibovitz
Mar 15, 2011·Diagnostic Microbiology and Infectious Disease·Francesco LuzzaroGiovanni Gesu
Jan 31, 2012·Infectious Disease Clinics of North America·Lindsay E Nicolle
Oct 2, 2013·Infection and Immunity·Miriam Korte-BerwangerSören G Gatermann
Nov 26, 2013·American Journal of Infection Control·Kelly R DanielsChristopher R Frei
Dec 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sargurunathan SubashchandraboseHarry L T Mobley
Nov 20, 2016·Journal of the American Geriatrics Society·Chelsie E ArmbrusterLona Mody

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Methods Mentioned

BETA
deamination
urine pool
PCR

Software Mentioned

GraphPad Prism
BLAST
PATRIC
TargeTron
GraphPad
Gen5

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