D-serine induces lipid and protein oxidative damage and decreases glutathione levels in brain cortex of rats

Brain Research
Lucila de B da SilvaM Wajner

Abstract

The present work investigated the in vitro effects of D-serine (D-Ser) on important parameters of oxidative stress in cerebral cortex of young rats. Our results show that D-Ser significantly induced lipid peroxidation, as determined by increase of thiobarbituric acid-reactive substances and chemiluminescence levels, as well as protein oxidative damage since carbonyl formation and sulfhydryl oxidation were enhanced by this amino acid. Furthermore, the addition of free radical scavengers significantly prevented D-Ser-induced lipid oxidative damage, suggesting that free radicals were involved in this effect. D-Ser also significantly diminished glutathione levels in cortical supernatants, decreasing therefore the major brain antioxidant defense. Finally, D-Ser oxidized a glutathione commercial solution in a medium devoid of brain supernatants, indicating that it behaved as a direct acting oxidant. In contrast, L-serine, L-alanine and L-threonine at concentrations as high as 5 mM did not significantly change chemiluminescence values, carbonyl content and GSH concentrations, implying a selective effect for D-serine. However, cortical supernatants exposed to 5 mM L-serine for different periods resulted in a gradual enhancement of TBA-...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·H Esterbauer, K H Cheeseman
Mar 1, 1966·Journal of Neurochemistry·A H NeimsJ D Smilack
Jan 1, 1994·Methods in Enzymology·A Z Reznick, L Packer
Jan 21, 1994·Brain Research·Y NagataT Maeda
Mar 15, 1997·Biochimica Et Biophysica Acta·K HamaseK Imai
Feb 20, 1998·Neuroscience Letters·F HayashiT Nishikawa
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·H WoloskerS H Snyder
May 23, 2001·Archives of Biochemistry and Biophysics·P EvelsonE A Lissi
Dec 26, 2001·Journal of Neurochemistry·M B BogdanovM F Beal
Feb 28, 2002·Brain Research·Cátia S RibeiroHerman Wolosker
Apr 18, 2002·Biochimica Et Biophysica Acta·Estefanía Méndez-AlvarezJosé Luis Labandeira-García
Feb 19, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Isabella Dalle-DonneRoberto Colombo
Mar 8, 2003·Amino Acids·J C González-HernándezA Saavedra-Molina
May 9, 2003·Journal of the Neurological Sciences·Masafumi OgawaMitsuru Kawai
Jul 28, 2004·Molecular Psychiatry·D C Javitt
Aug 3, 2004·Journal of Neuroinflammation·Sheng-Zhou WuSteven W Barger
Nov 13, 2004·The Journal of Biological Chemistry·Veronika N FoltynHerman Wolosker
Feb 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Paul M KimSolomon H Snyder
Apr 20, 2005·Journal of Neurochemistry·N StoyL G Darlington
Jun 9, 2005·Progress in Neuro-psychopharmacology & Biological Psychiatry·Kenji HashimotoMasaomi Iyo
Nov 23, 2005·Chemical Research in Toxicology·Masao MaekawaRyuichi Konno
Sep 22, 2006·Journal of Alzheimer's Disease : JAD·Michelangelo MancusoLuigi Murri
Nov 18, 2006·Toxicology·Marisol Orozco-IbarraJosé Pedraza-Chaverri
Dec 13, 2006·Schizophrenia Research·Inna BendikovGalila Agam
Dec 21, 2006·Topics in Magnetic Resonance Imaging : TMRI·Daniela Berg, Moussa B H Youdim
Sep 1, 2007·The EMBO Journal·Jumpei SasabeSadakazu Aiso

❮ Previous
Next ❯

Citations

Sep 30, 2009·Molecular Psychiatry·L VerrallP J Harrison
Feb 15, 2011·Drug and Chemical Toxicology·Guliz ArmaganAyfer Yalcin
Mar 24, 2010·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Guilhian LeipnitzMoacir Wajner
Jun 22, 2010·Chemistry & Biodiversity·Mendel Friedman
Sep 24, 2015·Pharmacological Reports : PR·Güliz ArmaganTaner Dagcı

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.