D-Xylose as a repressor or inducer of xylanase expression in Hypocrea jecorina (Trichoderma reesei).

Applied and Environmental Microbiology
Astrid R Mach-AignerRobert L Mach

Abstract

For Hypocrea jecorina (anamorph Trichoderma reesei), a filamentous fungus used for hydrolase production in different industries, it has been a long-term practice to use d-xylose as an inducing substance. We demonstrate in this study that the degree of xylanase-encoding gene induction strictly depends on the concentration of d-xylose, which was found to be optimal from 0.5 to 1 mM for 3 h of cultivation. At higher concentrations of d-xylose, a reduced level of xylanase gene expression was observed. In the present study, we also provide evidence that the d-xylose concentration-dependent induction is antagonized by carbon catabolite repressor 1. This repressor mediates its influence on d-xylose indirectly, by reducing the expression of xylanase regulator 1, the main activator of most hydrolase-encoding genes. Additionally, a direct influence of the repressor on xylanase 1 expression in the presence of d-xylose was found. Furthermore, we show that d-xylose reductase 1 is needed to metabolize d-xylose to achieve full induction of xylanase expression. Finally, a strain which expresses xylanase regulator 1 at a constant level was used to partially overcome the negative influence exerted by carbon catabolite repressor 1 on d-xylose.

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Citations

Jul 12, 2011·Applied and Environmental Microbiology·Astrid R Mach-AignerRobert L Mach
Feb 22, 2012·Applied and Environmental Microbiology·Astrid R Mach-AignerLeo H de Graaff
Jul 3, 2013·Annual Review of Microbiology·N Louise GlassSamuel Coradetti
Aug 31, 2010·Journal of Industrial Microbiology & Biotechnology·Oscar A Rojas-RejónTeresa Ponce-Noyola
Mar 31, 2015·Biochemical and Biophysical Research Communications·Zhen-Bang HuangZhi-Yang Dong
Aug 8, 2015·Biotechnology for Biofuels·Christian DerntlAstrid R Mach-Aigner
Jul 22, 2014·Fungal Genetics and Biology : FG & B·Mari ValkonenMojca Benčina
Jul 11, 2016·Trends in Biotechnology·Vijai Kumar GuptaRoberto N Silva
Jun 16, 2017·Biotechnology for Biofuels·Tiziano BenocciRonald P de Vries
Aug 29, 2018·Fungal biology and biotechnology·Alice RassingerAstrid R Mach-Aigner
Aug 9, 2020·Fungal biology and biotechnology·Christian DerntlAstrid Mach-Aigner
Jun 22, 2017·Scientific Reports·Thiago Rodrigues DutraSebastião Tavares de Rezende
Nov 9, 2018·Frontiers in Bioengineering and Biotechnology·Gustavo Pagotto BorinJuliana Velasco de Castro Oliveira
Oct 27, 2018·Frontiers in Bioengineering and Biotechnology·Elisabeth FitzBernhard Seiboth

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