D1-Asp170 is structurally coupled to the oxygen evolving complex in photosystem II as revealed by light-induced Fourier transform infrared difference spectroscopy

Biochemistry
H A ChuG T Babcock

Abstract

We report both mid-frequency (1800-1200 cm(-)(1)) and low-frequency (670-350 cm(-)(1)) S(2)/S(1) FTIR difference spectra of photosystem II (PSII) particles isolated from wild-type and D1-D170H mutant cells of the cyanobacterium Synechocystis sp. PCC 6803. Both mid- and low-frequency S(2)/S(1) spectra of the Synechocystis wild-type PSII particles closely resemble those from spinach PSII samples, which confirms an earlier result by Noguchi and co-workers [Noguchi, T., Inoue, Y., and Tang, X.-S. (1997) Biochemistry 36, 14705-14711] and indicates that the coordination environment of the oxygen evolving complex (OEC) in Synechocystis is very similar to that in spinach. We also found that there is no appreciable difference between the mid-frequency S(2)/S(1) spectra of wild-type and of D1-D170H mutant PSII particles, from which we conclude that D1-Asp170 does not undergo a significant structural change during the S(1) to S(2) transition. This result also suggests that, if D1-Asp170 ligates Mn, it does not ligate the Mn ion that is oxidized during the S(1) to S(2) state transition. Finally, we found that a mode at 606 cm(-)(1) in the low-frequency wild-type S(2)/S(1) spectrum shifts to 612 cm(-)(1) in the D1-D170H mutant spectrum. Bec...Continue Reading

References

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Citations

Nov 16, 2001·Phytochemical Analysis : PCA
May 17, 2008·Photosynthesis Research·Eduardo M SprovieroVictor S Batista
Oct 24, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Sonita SinghWarwick Hillier
Jul 14, 2011·Angewandte Chemie·Laure MarboutinCatherine Berthomieu
Feb 7, 2006·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Martina Wolpert, Petra Hellwig
Nov 10, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Manoj MandalM R Gunner
Apr 5, 2014·Physical Chemistry Chemical Physics : PCCP·Ravi Pokhrel, Gary W Brudvig
Aug 30, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Ipsita GhoshGary W Brudvig
Jun 28, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Gourab BanerjeeGary W Brudvig
Nov 10, 2006·Chemical Reviews·James P McEvoy, Gary W Brudvig

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