D1 dopamine receptor regulation of the levels of the cell-cycle-controlling proteins, cyclin D, P27 and Raf-1, in cerebral cortical precursor cells is mediated through cAMP-independent pathways

Cerebral Cortex
Ling ZhangM S Lidow

Abstract

Previously, we demonstrated that dopamine D1 receptor (D1R) agonists inhibit epidermal growth factor (EGF)-induced passage of mouse fetal cerebral cortical precursor cells from the G1 phase to the S phase of the cell cycle. Here, we report that this action of D1R agonists may involve regulation of cyclin D, and P27, which respectively promote and suppress the G1 to S transition. Furthermore, regulation of Raf-1, a component of the receptor tyrosine kinase mitogen-activated protein kinase pathway engaged in the mitogenic activity of EGF, may also be involved. Specifically, levels of cyclin D and Raf-1 decrease, whereas those of P27 first increase and then decrease in a dose-dependent fashion in response to the D1R agonist, SKF38393. This agonist also promotes Raf-1 phosphorylation on serine 338 residue, suggesting increased activation of this protein. Only the latter effect can be blocked by adenylyl cyclase (AC) and cAMP-dependent protein kinase A (PKA) inhibitors, and mimicked by agonists of the cAMP signaling pathway. Another D1R agonist, SKF83959, which stimulates phospholipase Cbeta (PLCbeta) but not AC, reduces levels of Raf-1 and cyclin D similar to SKF38393. However, we detected only down-regulation of P27 by this agonis...Continue Reading

Citations

Oct 10, 2006·Developmental Neuroscience·Kiyomi Y ArakiPradeep G Bhide
Apr 18, 2009·Developmental Neuroscience·Aliya L Frederick, Gregg D Stanwood
Aug 13, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Aiwu ChengJean C Shih
Apr 6, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·James E CrandallPradeep G Bhide
May 14, 2005·The Journal of Comparative Neurology·Gregg D StanwoodPat Levitt
Sep 28, 2005·Journal of Neurobiology·Ines Kralj-HansPeter Mobbs
Nov 10, 2015·Genes, Brain, and Behavior·G B Bissonette, M R Roesch
Dec 6, 2020·Neuroscience Letters·Yunyun CaiGang Chen
Jun 16, 2010·Pharmacology & Therapeutics·Ashiwel S Undieh

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