D471G mutation in LCMV-NP affects its ability to self-associate and results in a dominant negative effect in viral RNA synthesis

Viruses
Emilio Ortiz-RiañoLuis Martínez-Sobrido

Abstract

Arenaviruses merit significant interest because several family members are etiological agents of severe hemorrhagic fevers, representing a major burden to public health. Currently, there are no FDA-licensed vaccines against arenaviruses and the only available antiviral therapy is limited to the use of ribavirin that is partially effective. Arenavirus nucleoprotein (NP) is found associated with the genomic RNA forming the viral ribonucleoproteins (vRNPs) that together with the polymerase (L) direct viral replication and transcription. Virion formation requires the recruitment of vRNPs into budding sites, a process in which the arenavirus matrix-like protein (Z) plays a major role. Therefore, proper NP-NP and NP-Z interactions are required for the generation of infectious progeny. In this work we demonstrate the role of the amino acid residue D471 in the self-association of lymphocytic choriomeningitis virus nucleoprotein (LCMV-NP). Amino acid substitutions at this position abrogate NP oligomerization, affecting its ability to mediate replication and transcription of a minigenome reporter plasmid. However, its ability to interact with the Z protein, counteract the cellular interferon response and bind to dsRNA analogs was retaine...Continue Reading

References

Sep 1, 1988·Antimicrobial Agents and Chemotherapy·K T McKeeB G Mahlandt
Jan 1, 1986·Medical Microbiology and Immunology·R M ZinkernagelL Stitz
Jan 2, 1986·The New England Journal of Medicine·J B McCormickR Belmont-Williams
Mar 29, 1974·Deutsche medizinische Wochenschrift·R AckermannP Hochgesand
Apr 1, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P E KilgoreC J Peters
Oct 12, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M Isaäcson
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·O LenzW Garten
Apr 27, 2002·Acta Crystallographica. Section D, Biological Crystallography·Sven HovmöllerTomas Ohlson
May 4, 2002·Current Topics in Microbiology and Immunology·R M Zinkernagel
Mar 5, 2003·Antiviral Research·Rémi N Charrel, Xavier de Lamballerie
Oct 7, 2003·Structure·Markus G RudolphRalf Ficner
Oct 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mar PerezJuan C de la Torre
Sep 29, 2004·Journal of Virology·Mar PerezJuan Carlos de la Torre
Oct 19, 2004·Critical Reviews in Clinical Laboratory Sciences·Stephan Günther, Oliver Lenz
Mar 31, 2006·Journal of Virology·Shinji WatanabeYoshihiro Kawaoka
May 26, 2006·The New England Journal of Medicine·Staci A FischerUNKNOWN LCMV in Transplant Recipients Investigation Team
Jun 17, 2006·Science·Aurélie A V AlbertiniRob W H Ruigrok
Nov 7, 2006·Virology Journal·Thomas StreckerWolfgang Garten
Jun 20, 2007·Nucleic Acids Research·Alberto LabargaRodrigo Lopez
Sep 7, 2007·Journal of Virology·Luis Martínez-SobridoJuan Carlos de la Torre
Feb 8, 2008·The New England Journal of Medicine·Gustavo PalaciosW Ian Lipkin
Jul 11, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andy Ka-Leung NgPang-Chui Shaw
Feb 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sebastien F EmonetJuan C de la Torre
Apr 2, 2010·Journal of Virology·Kristine M RoseSusan R Weiss
Nov 19, 2010·Nature·Xiaoxuan QiChangjiang Dong
Dec 3, 2010·Journal of Virology·W W Shanaka I RodrigoLuis Martínez-Sobrido
Dec 17, 2010·Journal of Virology·Jesica M Levingston MacleodNora Lopez
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kathryn M HastieErica Ollmann Saphire

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Citations

Apr 5, 2013·Archives of Virology·Frank LennartzWolfgang Garten
Jun 11, 2014·Acta Crystallographica. Section D, Biological Crystallography·Brandyn R WestErica Ollmann Saphire
Feb 1, 2013·The Journal of General Virology·Emilio Ortiz-RiañoLuis Martínez-Sobrido
Jul 23, 2016·Viruses·Luis Martínez-Sobrido, Juan Carlos de la Torre
Jul 30, 2019·Current Opinion in Virology·Brett BeitzelGustavo Palacios
Feb 7, 2018·ACS Infectious Diseases·Pedro O MirandaJuan C de la Torre

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Datasets Mentioned

BETA
AB9484

Methods Mentioned

BETA
nuclear translocation
co-immunoprecipitation
two-hybrid
Co-IP
transfection
fluorescence microscopy
pull-down
PCR
electrophoresis
Pull

Software Mentioned

Excel
imageJ
EMBL
- CLUSTALW

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