Aug 6, 2015

DADA2: High resolution sample inference from amplicon data

BioRxiv : the Preprint Server for Biology
Benjamin J CallahanSusan P Holmes

Abstract

Microbial communities are commonly characterized by amplifying and sequencing target genes, but errors limit the precision of amplicon sequencing. We present DADA2, a software package that models and corrects amplicon errors. DADA2 identified more real variants than other methods in Illumina-sequenced mock communities, some differing by a single nucleotide, while outputting fewer spurious sequences. DADA2 analysis of vaginal samples revealed a diversity of Lactobacillus crispatus strains undetected by OTU methods.

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Mentioned in this Paper

Computer Software
Gene Amplification
Genes
Nucleic Acid Sequencing
Sequencing
Nucleotides
Microbial
Molecular Genetic Technique
Base Sequence
Analysis

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