Daily Gene Expression Rhythms in Rat White Adipose Tissue Do Not Differ Between Subcutaneous and Intra-Abdominal Depots

Frontiers in Endocrinology
Rianne van der SpekAndries Kalsbeek

Abstract

White adipose tissue (WAT) is present in different depots throughout the body. Although all depots are exposed to systemic humoral signals, they are not functionally identical. Studies in clock gene knockout animals and in shift workers suggest that daily rhythmicity may play an important role in lipid metabolism. Differences in rhythmicity between fat depots might explain differences in depot function; therefore, we measured mRNA expression of clock genes and metabolic genes on a 3-h interval over a 24-h period in the subcutaneous inguinal depot and in the intra-abdominal perirenal, epididymal, and mesenteric depots of male Wistar rats. We analyzed rhythmicity using CircWave software. Additionally, we measured plasma concentrations of glucose, insulin, corticosterone, and leptin. The clock genes (Bmal1/Per2/Cry1/Cry2/RevErbα/DBP) showed robust daily gene expression rhythms, which did not vary between WAT depots. Metabolic gene expression rhythms (SREBP1c/PPARα/PPARγ/FAS/LPL/Glut4/HSL/CPT1b/leptin/visfatin/resistin) were more variable between depots. However, no distinct differences between intra-abdominal and subcutaneous rhythms were found. Concluding, specific fat depots are not associated with differences in clock gene expr...Continue Reading

References

Aug 12, 1998·The Journal of Steroid Biochemistry and Molecular Biology·B DesvergneW Wahli
Aug 14, 1999·Journal of Neuroendocrinology·S E La FleurR M Buijs
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M J SweetF Y Liew
Aug 4, 2001·Pflügers Archiv : European journal of physiology·P OliverA Palou
Mar 6, 2003·Neuroscience Letters·Christian RamakersAntoon F M Moorman
Apr 30, 2003·The American Journal of Clinical Nutrition·Marieke B SnijderJacob C Seidell
May 15, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robert R BowersTimothy J Bartness
Mar 23, 2005·Biochemical and Biophysical Research Communications·Willem KamphuisRuud M Buijs
Apr 23, 2005·Science·Fred W TurekJoseph Bass
Mar 29, 2006·Diabetes·Sanjin ZvonicJeffrey M Gimble
Sep 22, 2006·Human Molecular Genetics·Caroline H Ko, Joseph S Takahashi
Feb 16, 2007·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·M S Bray, M E Young
Nov 7, 2007·Cell Metabolism·Akira KohsakaJoseph Bass
Feb 25, 2009·Nucleic Acids Research·J M RuijterA F M Moorman
Apr 24, 2009·Clinical and Experimental Hypertension : CHE·Hitoshi AndoAkio Fujimura
May 30, 2009·Obesity·Cecilia Gómez-SantosMarta Garaulet
Oct 20, 2009·Diabetes Care·Shuichiro YamamotoTetsuya Mizoue
Nov 17, 2009·Metabolism: Clinical and Experimental·Reiner WiestAndreas Schäffler
Jun 15, 2011·Molecular and Cellular Endocrinology·Jonathan D Johnston
Mar 20, 2012·Trends in Endocrinology and Metabolism : TEM·Clara B PeekJoseph Bass
Sep 20, 2012·JAMA : the Journal of the American Medical Association·Ian J NeelandJames A de Lemos
Nov 13, 2012·Nature Medicine·Georgios K PaschosGarret A Fitzgerald
Feb 26, 2013·Diabetes·Anton ShostakHenrik Oster
Apr 16, 2013·Cell Metabolism·Tamara TchkoniaJames L Kirkland
May 21, 2013·Biochimica Et Biophysica Acta·Ursula A White, Yourka D Tchoukalova
Apr 12, 2014·Biochimica Et Biophysica Acta·Sander Kersten
Apr 17, 2014·Frontiers in Neuroendocrinology·Timothy J BartnessVitaly Ryu
Oct 19, 2014·Journal of Biological Rhythms·Paul F Thaben, Pål O Westermark
Jan 30, 2015·Journal of Pineal Research·Talita da Silva Mendes de FariasFabio Bessa Lima
Dec 24, 2015·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·J Laermans, I Depoortere
Mar 1, 2017·Frontiers in Endocrinology·Anthony H TsangHenrik Oster

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Citations

Sep 5, 2020·Journal of Biological Rhythms·Tatienne Neder Figueira da CostaPaula Bargi-Souza
Sep 4, 2020·Nature Communications·Lauren M HablitzMaiken Nedergaard
Nov 6, 2020·Endocrine Reviews·Sandra PereiraTimothy J Kieffer

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Methods Mentioned

BETA
gene knockout
biopsy
Biosen
PCR
electrophoresis
PCRs

Software Mentioned

CIRCWAVE
LinRegPCR

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