Daily supplementation with ghrelin improves in vitro bovine blastocysts formation rate and alters gene expression related to embryo quality

Theriogenology
Eleni DovolouGeorgios S Amiridis

Abstract

Ghrelin is a gastric peptide having regulatory role in the reproductive system functionality, acting mainly at central level. Because the expression of ghrelin system (ghrelin and its receptor) has been detected in the bovine ovary, the objectives of the present study were to investigate whether ghrelin can affect the developmental potential of in vitro-produced embryos, and to test their quality in terms of relative abundance of various genes related to metabolism, apoptosis and oxidation. In the first experiment, in vitro-produced zygotes were cultured in the absence (control [C]) and in the presence of three concentrations of acylated ghrelin (200 pg/mL [Ghr200], 800 pg/mL [Ghr800]; and 2000 pg/mL [Ghr2000]); blastocyst formation rates were examined on Days 7, 8, and 9. In the second experiment, only the 800 pg/mL dose of ghrelin was used. Zygotes were produced as in experiment 1 and 24 hours post insemination they were divided into 4 groups; in two groups (C; without ghrelin; Ghr800 with ghrelin), embryos were cultured without medium replacement; in the remaining two groups (Control N and GhrN), the culture medium was daily renewed. A pool of Day-7 blastocysts were snap frozen for relative mRNA abundance of various genes re...Continue Reading

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May 15, 2013·Theriogenology·Eleni DovolouGeorgios S Amiridis

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Citations

Jan 19, 2016·Journal of Assisted Reproduction and Genetics·George AnifandisIoannis E Messinis
Jun 4, 2014·Reproduction in Domestic Animals = Zuchthygiene·E DovolouG S Amiridis
Jun 23, 2016·Theriogenology·T M ChouzourisG S Amiridis
Jun 12, 2016·General and Comparative Endocrinology·Hen HonigEran Gershon
Aug 23, 2016·International Journal of Molecular Sciences·Yi-Ning ChaoYuh-Lin Wu
Mar 8, 2017·Zygote : the Biology of Gametes and Early Embryos·Thomas-Markos ChouzourisGeorgios S Amiridis
Sep 2, 2018·Reproduction in Domestic Animals = Zuchthygiene·Thomas Markos ChouzourisGeorgios S Amiridis
Sep 25, 2017·Endocrine Reviews·Luba SominskySarah J Spencer
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Konstantina StampernaGeorgios S Amiridis

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