Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

Biotechnology for Biofuels
Jan KolekPetra Patáková

Abstract

Butanol is currently one of the most discussed biofuels. Its use provides many benefits in comparison to bio-ethanol, but the price of its fermentative production is still high. Genetic improvements could help solve many problems associated with butanol production during ABE fermentation, such as its toxicity, low concentration achievable in the cultivation medium, the need for a relatively expensive substrate, and many more. Clostridium pasteurianum NRRL B-598 is non-type strain producing butanol, acetone, and a negligible amount of ethanol. Its main benefits are high oxygen tolerance, utilization of a wide range of carbon and nitrogen sources, and the availability of its whole genome sequence. However, there is no established method for the transfer of foreign DNA into this strain; this is the next step necessary for progress in its use for butanol production. We have described functional protocols for conjugation and transformation of the bio-butanol producer C. pasteurianum NRRL B-598 by foreign plasmid DNA. We show that the use of unmethylated plasmid DNA is necessary for efficient transformation or successful conjugation. Genes encoding DNA methylation and those for restriction-modification systems and antibiotic resistan...Continue Reading

References

Feb 1, 1990·Agricultural and Biological Chemistry·S YoshinoS Hayashida
Sep 1, 1988·Applied and Environmental Microbiology·S P Allen, H P Blaschek
Nov 1, 1971·Journal of General Microbiology·R W O'Brien, J G Morris
Jul 1, 1983·Journal of Bacteriology·N P Minton, J G Morris
Aug 1, 1995·The Journal of Applied Bacteriology·I R McDonaldA J McCarthy
Apr 18, 1998·Applied and Environmental Microbiology·N B FitzgeraldT J Vanden Boom
Oct 1, 1984·Applied and Environmental Microbiology·Y L Lin, H P Blaschek
Sep 25, 2007·Nucleic Acids Research·Yizhi SongWei E Huang
May 19, 2009·Journal of Microbiological Methods·John T HeapNigel P Minton
Jun 30, 2009·Journal of Industrial Microbiology & Biotechnology·Yan-Ning ZhengDong-Zhi He
Nov 7, 2009·Journal of Microbiological Methods·John T HeapNigel P Minton
May 11, 2010·Nature Methods·Benjamin A FlusbergStephen W Turner
May 9, 2012·Biotechnology for Biofuels·Adam M GussLee R Lynd
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Kommireddy Vasu, Valakunja Nagaraja
Apr 11, 2013·Biotechnology for Biofuels·Michael E PyneC Perry Chou
May 8, 2013·Antimicrobial Agents and Chemotherapy·Andrew G McArthurGerard D Wright
Apr 29, 2014·Biotechnology Advances·Michael E PyneC Perry Chou

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Citations

May 29, 2016·Anaerobe·Nigel P MintonYing Zhang
Feb 2, 2018·Journal of Basic Microbiology·Yayun WangKazuo Sakka
May 12, 2018·Biotechnology and Bioengineering·Emily FreedCarrie A Eckert
Aug 19, 2018·Microbial Cell Factories·Megumi MiyanoKen-Ichi Yoshida
Oct 23, 2019·Biotechnology for Biofuels·Karel SedlarIvo Provaznik
Jul 31, 2019·Methods : a Companion to Methods in Enzymology·Mamou DialloAna M López-Contreras
Jan 27, 2021·Biotechnology for Biofuels·Lauren A Riley, Adam M Guss
Jan 8, 2021·Applied Microbiology and Biotechnology·Barbora BranskaPetra Patakova
Feb 18, 2021·FEMS Microbiology Reviews·Barbara BourgadeM Ahsanul Islam
Dec 1, 2017·ACS Synthetic Biology·Ivan S GyulevGavin H Thomas
Jul 15, 2021·Applied and Environmental Microbiology·Rudolf O SchlechterMitja N P Remus-Emsermann
Nov 19, 2021·Journal of Microbiological Methods·Takamitsu AraiAkihiko Kosugi

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Datasets Mentioned

BETA
ALB45592
GM33

Methods Mentioned

BETA
chemical treatment
genetic modification
electrophoresis

Software Mentioned

REBASE
BLAST
SMRT Analysis
ClosTron
R
Matlab

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