Damage to lens fiber cells causes TRPV4-dependent Src family kinase activation in the epithelium

Experimental Eye Research
Mohammad ShahidullahNicholas A Delamere

Abstract

The bulk of the lens consists of tightly packed fiber cells. Because mature lens fibers lack mitochondria and other organelles, lens homeostasis relies on a monolayer of epithelial cells at the anterior surface. The detection of various signaling pathways in lens epithelial cells suggests they respond to stimuli that influence lens function. Focusing on Src Family Kinases (SFKs) and Transient Receptor Potential Vanilloid 4 (TRPV4), we tested whether the epithelium can sense and respond to an event that occurs in fiber mass. The pig lens was subjected to localized freeze-thaw (FT) damage to fibers at posterior pole then the lens was incubated for 1-10 min in Krebs solution at 37 °C. Transient SFK activation in the epithelium was detectable at 1 min. Using a western blot approach, the ion channel TRPV4 was detected in the epithelium but was sparse or absent in fiber cells. Even though TRPV4 expression appears low at the actual site of FT damage to the fibers, SFK activation in the epithelium was suppressed in lenses subjected to FT damage then incubated with the TRPV4 antagonist HC067047 (10 μM). Na,K-ATPase activity was examined because previous studies report changes of Na,K-ATPase activity associated with SFK activation. Na,K-...Continue Reading

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Citations

Oct 19, 2016·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Nicholas A DelamereMohammad Shahidullah
Aug 18, 2016·European Journal of Cell Biology·María Macarena Massip Copiz, Tomás Antonio Santa Coloma
Dec 15, 2017·Cephalalgia : an International Journal of Headache·Fan BuMinyan Wang
Sep 13, 2018·American Journal of Physiology. Cell Physiology·Mohammad ShahidullahNicholas A Delamere
Jun 3, 2016·Physiological Reviews·John P M WhiteIstvan Nagy
Apr 16, 2020·American Journal of Physiology. Cell Physiology·Mohammad ShahidullahNicholas A Delamere
Apr 15, 2018·Experimental Eye Research·Amritlal MandalNicholas A Delamere
Sep 27, 2020·Experimental Eye Research·Brianna D GuarinoCharles K Thodeti

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