Abstract
The Drosophila follicular epithelium, which surrounds developing egg chambers, is a well-established model for studying epithelial polarity because it is continuously generated from adult stem cells, making it easy to generate homozygous mutant clones in a heterozygous background. Mutant clones are usually marked by the loss of Green Fluorescent Protein (GFP) expression, which distinguishes them from their green, wild-type neighbours. Here we report that damage to the epithelium during dissection can produce groups of GFP-negative cells that resemble mutant clones. Furthermore, several polarity factors, such as aPKC and Discs large, are not localised in these damage-induced false clones. This phenotype is identical to that reported for several mutants, including ampk and Dystroglycan mutant clones under conditions of energetic stress. Using more reliable systems to mark ampk and Dystroglycan null clones such as the MARCM system, we found that neither protein is required for epithelial polarity under low energy conditions. Thus, our previous report of a specific low energy polarity pathway is an artefact of the increased damage caused by dissecting the small ovaries of starved flies. However, ampk mutant cells are larger than no...Continue Reading
References
Apr 1, 1984·Environmental Health Perspectives·J S Bus, J E Gibson
Aug 12, 1998·Developmental Biology·R I Woodruff, L G Tilney
Mar 3, 1999·Cell·N Ito, G M Rubin
Apr 10, 1999·Neuron·T Lee, L Luo
Jun 10, 2000·Cell·T P NewsomeB J Dickson
Jul 7, 2000·Science·D BilderN Perrimon
Nov 15, 2000·The Journal of Cell Biology·G TanentzapfU Tepass
Feb 22, 2001·Developmental Biology·D Drummond-Barbosa, A C Spradling
May 12, 2001·Cell·N TaponI K Hariharan
May 12, 2001·Cell·C J PotterT Xu
Jun 8, 2001·Genes & Development·X Gao, D Pan
Jan 24, 2003·Nature·Sophie G Martin, Daniel St Johnston
Jul 23, 2003·Development·Hélène DoerflingerDaniel St Johnston
Dec 4, 2003·Cell·Ken InokiKun-Liang Guan
Dec 17, 2003·Cell·Richard Benton, Daniel St Johnston
Mar 27, 2007·Developmental Biology·Ryan ConderNicholas Harden
Apr 5, 2007·Nature Protocols·Joy S Wu, Liqun Luo
May 2, 2007·The Journal of Cell Biology·Vincent MirouseJay E Brenman
May 9, 2007·Nature·Jun Hee LeeJongkyeong Chung
Dec 21, 2007·Developmental Biology·Christina P ChristoforouRobert P Ray
Dec 17, 2008·Current Biology : CB·Rebecca Bastock, Daniel St Johnston
Jan 22, 2009·Developmental Cell·Vincent MirouseRobert P Ray
May 4, 2010·Cell·Eurico Morais-de-SáDaniel St Johnston
Jun 1, 2010·Cell·Daniel St Johnston, Julie Ahringer
Jun 4, 2010·Cell Metabolism·Simon A HawleyD Grahame Hardie
Jun 25, 2010·Development·Pei SunRongwen Xi
Dec 3, 2011·Journal of Cell Science·Stephanie J AiroldiLynn Cooley
Mar 17, 2012·Biochimica Et Biophysica Acta·Bruce H Cohen, Russell P Saneto
Mar 23, 2012·Nature Reviews. Molecular Cell Biology·D Grahame HardieSimon A Hawley
Aug 3, 2012·Molecular Biology of the Cell·Alexandre D BaffetAntoine Guichet
Oct 30, 2013·The Journal of Cell Biology
Citations
Oct 30, 2013·The Journal of Cell Biology
Jul 23, 2014·Developmental Biology·Valérie BelloThierry Darribère
May 20, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tsung-Han YehJenn-Yah Yu
Jul 12, 2014·Current Opinion in Cell Biology·Alejo E Rodriguez-Fraticelli, Fernando Martin-Belmonte
May 8, 2016·Developmental Biology·Ryan ConderNicholas Harden
Dec 2, 2014·ELife·Angela CastanietoTodd G Nystul
Oct 21, 2016·Developmental Biology·Kaitlin M Laws, Daniela Drummond-Barbosa
Nov 5, 2016·ELife·Nicolas AznarPradipta Ghosh
Nov 3, 2017·Nature Communications·Wei DaiDenise J Montell
Mar 22, 2014·Nature·Virginia Gewin
Dec 1, 2019·Development·Holly E LovegroveDaniel St Johnston
Feb 18, 2017·Aging·Pradipta Ghosh
Aug 29, 2019·Annual Review of Cell and Developmental Biology·Melissa A PickettJessica L Feldman
Sep 13, 2019·ELife·Jelle van den Ameele, Andrea H Brand
Jul 24, 2014·Development·Lara C SkwarekDavid Bilder