Damage to the Drosophila follicle cell epithelium produces "false clones" with apparent polarity phenotypes

Biology Open
Timm HaackDaniel St Johnston

Abstract

The Drosophila follicular epithelium, which surrounds developing egg chambers, is a well-established model for studying epithelial polarity because it is continuously generated from adult stem cells, making it easy to generate homozygous mutant clones in a heterozygous background. Mutant clones are usually marked by the loss of Green Fluorescent Protein (GFP) expression, which distinguishes them from their green, wild-type neighbours. Here we report that damage to the epithelium during dissection can produce groups of GFP-negative cells that resemble mutant clones. Furthermore, several polarity factors, such as aPKC and Discs large, are not localised in these damage-induced false clones. This phenotype is identical to that reported for several mutants, including ampk and Dystroglycan mutant clones under conditions of energetic stress. Using more reliable systems to mark ampk and Dystroglycan null clones such as the MARCM system, we found that neither protein is required for epithelial polarity under low energy conditions. Thus, our previous report of a specific low energy polarity pathway is an artefact of the increased damage caused by dissecting the small ovaries of starved flies. However, ampk mutant cells are larger than no...Continue Reading

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Citations

Jul 23, 2014·Developmental Biology·Valérie BelloThierry Darribère
May 20, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tsung-Han YehJenn-Yah Yu
Jul 12, 2014·Current Opinion in Cell Biology·Alejo E Rodriguez-Fraticelli, Fernando Martin-Belmonte
Oct 21, 2016·Developmental Biology·Kaitlin M Laws, Daniela Drummond-Barbosa
Mar 22, 2014·Nature·Virginia Gewin
Dec 1, 2019·Development·Holly E LovegroveDaniel St Johnston
Aug 29, 2019·Annual Review of Cell and Developmental Biology·Melissa A PickettJessica L Feldman

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Methods Mentioned

BETA
dissection
PCR

Software Mentioned

ImageJ
LaserGene SeqMan Pro
MARCM

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