Damaged DNA-binding protein 2 accelerates UV-damaged DNA repair in human corneal endothelium

Experimental Eye Research
Taeko InokiSatoru Yamagami

Abstract

To determine damaged DNA-binding protein 2-gene expression levels in vitro and ex vivo, and the degree of DNA repair in damaged DNA-binding protein 2-overexpressing cultured human corneal endothelium after ultraviolet irradiation. Constitutive damaged DNA-binding protein 2-gene expression levels in various human tissues were determined by semi-quantitative reverse transcription-polymerase chain reactions. The dynamics of nucleotide excision repair-related gene expression in cultured human corneal endothelium were investigated in a ribonuclease protection assay after ultraviolet-irradiation. The effect of damaged DNA-binding protein 2 on DNA repair was studied after ultraviolet-irradiation in cultured human corneal endothelium infected with adenovirus carrying damaged DNA-binding protein 2. Human corneal endothelium and epithelium in the donor cornea had the highest constitutive damaged DNA-binding protein 2-gene expression of the various human tissues studied. Gene expression level dynamics associated with nucleotide excision repair factors after ultraviolet-irradiation showed that the increase in the rate of damaged DNA-binding protein 2-gene expression in cultured human corneal endothelium was highest of the nucleotide excisi...Continue Reading

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Citations

Sep 5, 2008·The Journal of Investigative Dermatology·Melanie StruweUlla Plappert-Helbig
Aug 30, 2014·The Tohoku Journal of Experimental Medicine·Ewelina SynowiecJacek P Szaflik
Oct 19, 2006·Ophthalmic Research·Seiichi YokooTsuyoshi Takato
Jul 8, 2005·Current Opinion in Ophthalmology·Gerami D Seitzman

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