Danhong injection protects cardiomyocytes against hypoxia/reoxygenation- and H2O2-induced injury by inhibiting mitochondrial permeability transition pore opening

Journal of Ethnopharmacology
Zhen-Zhen DuanXiu-Mei Gao

Abstract

Danhong injection (DHI), a Chinese medical product extracted from Radix et Rhizoma Salviae Miltiorrhizae (Salvia miltiorrhiza Bge., Labiatae, Danshen in Chinese) and Flos Carthami (Carthamus tinctorius L., Compositae, Honghua in Chinese), has been widely used for the treatment of ischemic heart disease, and clinical and experimental studies have demonstrated the protective effects against myocardial ischemia/reperfusion injury. Nevertheless, the underlying cellular mechanisms responsible for this protective effect are poorly understood. The present study aimed to examine the mechanism of DHI in regulating hypoxia/reoxygenation- and H2O2-induced cardiomyocytes injury. Neonatal rat cardiomyocytes were subjected to hypoxia (9h)-reoxygenation (2h) or H2O2 (100 μM) in the presence or absence of DHI (2.5, 5, 10 μg/mL). Intracellular reactive oxygen species (ROS), cytosolic and mitochondrial Ca(2+) concentrations, mitochondrial membrane potential (ΔΨm) and mitochondrial permeability transition pore (mPTP) opening were monitored using CMH2DCFDA, Fluo-4 and rhod-2, JC-1 and calcein, respectively. Cell survival was evaluated using the 2-(4,5-dimethylthiazol-2-yl)-2,5 -diphenyltetrazolium bromide (MTT) assay and apoptosis was detected by ...Continue Reading

References

May 23, 2002·Biochimie·Anne-Sophie BelzacqCatherine Brenner
Sep 5, 2002·Free Radical Biology & Medicine·Paul S BrookesVictor M Darley-Usmar
Feb 14, 2004·Cardiovascular Research·Andrew P HalestrapSabzali A Javadov
Nov 13, 2004·Circulation Research·Michael T CrowRichard N Kitsis
May 10, 2007·The Journal of Biological Chemistry·Emmanuel RobinPaul T Schumacker
Dec 7, 2007·The New England Journal of Medicine·Radhakrishnan Ramaraj
Apr 9, 2008·Physiological Reviews·Elizabeth Murphy, Charles Steenbergen
Feb 27, 2009·Basic Research in Cardiology·Derek J HausenloyDerek M Yellon
Dec 15, 2010·Chemico-biological Interactions·Brian E SansburyBradford G Hill
Feb 26, 2011·Science·Enzo R PorrelloHesham A Sadek
Jan 14, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jae-Sung KimJohn J Lemasters
Sep 27, 2012·Journal of Ethnopharmacology·Yu HePanke Xing
Dec 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Enzo R PorrelloHesham A Sadek
Jan 19, 2013·Journal of Molecular and Cellular Cardiology·Tayyab ShahzadYaser Abdallah
Sep 10, 2013·Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·Bin-Jun YanHai-Bin Qu
Sep 18, 2013·Amino Acids·Silvia GrancaraAntonio Toninello
Apr 8, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Zefeng WangHong Zhang

❮ Previous
Next ❯

Citations

Jul 28, 2016·Journal of Pharmaceutical and Biomedical Analysis·Long GuoPing Li
Jul 10, 2019·Restorative Neurology and Neuroscience·John Owoicho OrgahYan Zhu
Feb 26, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Xue-Bin CaoYang Li
Jan 30, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Jingjing ZhangHongjun Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis