Danshensu has anti-tumor activity in B16F10 melanoma by inhibiting angiogenesis and tumor cell invasion

European Journal of Pharmacology
Li-juan ZhangAi-yun Wang

Abstract

Danshensu, the major water-soluble component of Radix Salviae Miltiorrhizae (Danshen), is the basic chemical structure of various salvianolic acids. This study was to evaluate the anti-tumor activity of danshensu in a series of in vitro and in vivo models. The effect of danshensu on B16F10 melanoma cell and HUVEC proliferation were assessed by MTS assay, and cell invasion and migration were investigated by transwell chamber assay. The effect of danshensu on angiogenesis was evaluated by HUVEC migration assay, tube formation assay and chick chorioallantoic membrane assay. The expression of MMP-2, -9 and VEGF in B16F10 melanoma cell were detected by western blotting after danshensu treatment. The role of danshensu in tumor metastasis in vivo was evaluated by spontaneous and experimental B16F10 melanoma metastasis model. Although danshensu had no inhibitory effect on B16F10 melanoma cell and HUVEC proliferation, it significantly inhibited B16F10 melanoma cell invasion (at 0.05, 0.5, 5 microM) and migration (at 0.5, 5 microM). It also dramatically suppressed VEGF-induced endothelial migration (at 0.5, 5 microM), tube formation in vitro (at 4, 20 microM) and new vessel formation in CAM in vivo (100 microg/egg). Danshensu (at 5, 50 m...Continue Reading

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Citations

Oct 18, 2012·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Li ZhangGuang Ji
Mar 13, 2014·Journal of Industrial Microbiology & Biotechnology·Chunying LiuFengxie Jin
Oct 15, 2013·Medicinal Research Reviews·Xiuping ChenYitao Wang
Jun 12, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Li TaoYin Lu
Jan 22, 2013·Experimental Cell Research·Carla ZimmermanCalvin D Roskelley
Mar 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenyi LiangLanzhen Zhang
Mar 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaodan ZhangKuancheng Liu
Feb 18, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Shao-Jun Chen, Ji-Long Ren
Jun 13, 2019·Applied Microbiology and Biotechnology·Tianzhen XiongYujie Cai
May 11, 2018·Critical Reviews in Food Science and Nutrition·Min ShiGuoyin Kai
Dec 24, 2019·European Journal of Pharmacology·Jinjing JiaDancan Chen
Feb 2, 2021·Frontiers in Pharmacology·Shao-Hsiang LiuAlan Yueh-Luen Lee

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