Dansyl-Labelled Ag@SiO2 Core-Shell Nanostructures-Synthesis, Characterization, and Metal-Enhanced Fluorescence

Materials
Elżbieta SzczepańskaBeata Grobelna

Abstract

The present work describes synthesis, characterization, and use of a new dansyl-labelled Ag@SiO2 nanocomposite as an element of a new plasmonic platform to enhance the fluorescence intensity. Keeping in mind that typical surface plasmon resonance (SPR) characteristics of silver nanoparticles coincide well enough with the absorption of dansyl molecules, we used them to build the core of the nanocomposite. Moreover, we utilized 10 nm amino-functionalized silica shell as a separator between silver nanoparticles and the dansyl dye to prevent the dye-to-metal energy transfer. The dansyl group was incorporated into Ag@SiO2 core-shell nanostructures by the reaction of aminopropyltrimethoxysilane with dansyl chloride and we characterized the new dansyl-labelled Ag@SiO2 nanocomposite using transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR). Additionally, water wettability measurements (WWM) were carried out to assess the hydrophobicity and hydrophilicity of the studied surface. We found that the nanocomposite deposited on a semitransparent silver mirror strongly increased the fluorescence intensity of dansyl dye (about 87-fold) compared with the control sample on the glass, proving that the system ...Continue Reading

References

Jan 1, 1970·Methods of Biochemical Analysis·N Seiler
Oct 25, 2001·Analytical Biochemistry·J R Lakowicz
Apr 4, 2003·Analytical Biochemistry·Joanna MalickaJoseph R Lakowicz
Jul 23, 2003·Photochemistry and Photobiology·Joseph R LakowiczIgnacy Gryczynski
Dec 25, 2004·Journal of Fluorescence·Joanna LukomskaJoseph R Lakowicz
Dec 25, 2004·Journal of Fluorescence·Joseph R LakowiczJun Huang
Sep 1, 2006·The Journal of Physical Chemistry. B·Shanlin PanLewis J Rothberg
Mar 13, 2009·Chemical Physics Letters·Evgenia G MatveevaZygmunt Gryczynski
Aug 12, 2009·Applied Microbiology and Biotechnology·Wen-Ru LiYi-Ben Chen
Dec 1, 2009·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Kadir AslanChris D Geddes
Jul 26, 2011·The Journal of Physical Chemistry. B·Piotr BojarskiSabato D'Auria
Oct 5, 2011·Chemical Communications : Chem Comm·Jianping YangDongyuan Zhao
Mar 7, 2012·Nanoscale·Nan Gao, Yuying Yan
Mar 21, 2013·Journal of Molecular Modeling·Katarzyna Walczewska-SzewcSabato d'Auria
Nov 26, 2013·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Tomasz GrzybStefan Lis
Dec 6, 2012·The Journal of Physical Chemistry Letters·Simeonika Rangełowa-JankowskaPiotr Bojarski
Oct 21, 2015·Journal of the American Chemical Society·Chao-Yu LiZhong-Qun Tian
Aug 1, 2019·Angewandte Chemie·Guangze YangChun-Xia Zhao
Sep 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elżbieta SzczepańskaPaweł Niedziałkowski

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Methods Mentioned

BETA
biosensing
surface plasmon resonance
transmission electron microscopy

Software Mentioned

FluoFit Pro

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