Dantrolene Ameliorates Impaired Neurogenesis and Synaptogenesis in Induced Pluripotent Stem Cell Lines Derived from Patients with Alzheimer's Disease.

Anesthesiology
Yong WangHuafeng Wei

Abstract

Overactivation of ryanodine receptors and the resulting impaired calcium homeostasis contribute to Alzheimer's disease-related pathophysiology. This study hypothesized that exposing neuronal progenitors derived from induced pluripotent stems cells of patients with Alzheimer's disease to dantrolene will increase survival, proliferation, neurogenesis, and synaptogenesis. Induced pluripotent stem cells obtained from skin fibroblast of healthy subjects and patients with familial and sporadic Alzheimer's disease were used. Biochemical and immunohistochemical methods were applied to determine the effects of dantrolene on the viability, proliferation, differentiation, and calcium dynamics of these cells. Dantrolene promoted cell viability and proliferation in these two cell lines. Compared with the control, differentiation into basal forebrain cholinergic neurons significantly decreased by 10.7% (32.9 ± 3.6% vs. 22.2 ± 2.6%, N = 5, P = 0.004) and 9.2% (32.9 ± 3.6% vs. 23.7 ± 3.1%, N = 5, P = 0.017) in cell lines from sporadic and familial Alzheimer's patients, respectively, which were abolished by dantrolene. Synapse density was significantly decreased in cortical neurons generated from stem cells of sporadic Alzheimer's disease by 58...Continue Reading

References

May 14, 2003·Neurochemistry International·Dorota MakarewiczJerzy W Łazarewicz
Sep 15, 2004·Journal of Chemical Neuroanatomy·Taiichi KatayamaMasaya Tohyama
May 12, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Grace E StutzmannIan Parker
Jul 12, 2007·Autophagy·Francesco CecconiMauro Piacentini
Mar 9, 2010·Journal of Neuroscience Research·Michael DemarsOrly Lazarov
Jun 25, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hua ZhangIlya Bezprozvanny
Sep 10, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ivan GoussakovGrace E Stutzmann
Sep 24, 2010·Anesthesia and Analgesia·Saadet Inan, Huafeng Wei
Oct 19, 2010·Biochimica Et Biophysica Acta·Simonetta Camandola, Mark P Mattson
Dec 15, 2010·Frontiers in Neuroscience·Jun Ming Wang, Chenyou Sun
May 3, 2011·Neurobiology of Aging·Angela M BrunoGrace E Stutzmann
May 17, 2011·Reviews in the Neurosciences·Karim BordjiAlain Buisson
Jul 26, 2011·Science China. Life Sciences·Shreaya Chakroborty, Grace E Stutzmann
Jan 27, 2012·Nature·Mason A IsraelLawrence S B Goldstein
Aug 24, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bénédicte OulèsMounia Chami
Jun 5, 2014·Frontiers in Bioscience (Landmark Edition)·Michael Allgaier, Clemens Allgaier
Jan 1, 2015·Alzheimer Disease and Associated Disorders·Li Liang, Huafeng Wei
Feb 5, 2015·Alzheimer Disease and Associated Disorders·Zhen WuHuafeng Wei
Apr 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Riccardo FiladiPaola Pizzo
May 1, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shreaya ChakrobortyGrace E Stutzmann
Apr 7, 2016·Annual Review of Neuroscience·Carlo Sala Frigerio, Bart De Strooper
May 21, 2016·Frontiers in Cellular Neuroscience·Kyung Min ChungSeong-Woon Yu
Nov 20, 2016·Neurotoxicology and Teratology·Meirong Yang, Huafeng Wei
Mar 23, 2017·Biochemical Pharmacology·Tim VervlietGeert Bultynck
May 5, 2017·Nature Reviews. Neuroscience·Christoph Anacker, René Hen
Aug 15, 2017·Psychological Medicine·J P Robbins, J Price
Dec 8, 2017·Journal of Alzheimer's Disease & Parkinsonism·Yong WangHuafeng Wei

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Citations

Jul 6, 2020·Journal of Alzheimer's Disease : JAD·Yun ShiHuafeng Wei
Aug 21, 2021·Frontiers in Cell and Developmental Biology·Shanshan ChenDi Wang

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Datasets Mentioned

BETA
GM24675

Methods Mentioned

BETA
protein assay
electrophoresis

Software Mentioned

GraphPad Prism
Image J
IPLab
iVision

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