Dark quenched matrix metalloproteinase fluorogenic probe for imaging osteoarthritis development in vivo

Bioconjugate Chemistry
Seulki LeeKuiwon Choi

Abstract

The early detection of osteoarthritis (OA) is currently a key challenge in the field of rheumatology. Biochemical studies of OA have indicated that matrix metalloproteinase-13 (MMP-13) plays a central role in cartilage degradation. In this study, we describe the potential use of a dark-quenched fluorogenic MMP-13 probe to image MMP-13 in both in vitro and rat models. The imaging technique involved using a MMP-13 peptide substrate, near-infrared (NIR) dye, and a NIR dark quencher. The results from this study demonstrate that the use of a dark-quenched fluorogenic probe allows for the visual detection of MMP-13 in vitro and in OA-induced rat models. In particular, by targeting this OA biomarker, the symptoms of the early and late stages of OA can be readily monitored, imaged, and analyzed in a rapid and efficient fashion. We anticipate that this simple and highly efficient fluorogenic probe will assist in the clinical management of patients with OA, not only for early diagnosis but also to assess individual patient responses to new drug treatments.

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Citations

Aug 1, 2009·Bioconjugate Chemistry·Anca Dragulescu-AndrasiJianghong Rao
Jan 28, 2010·Biochemistry·Seulki LeeXiaoyuan Chen
Mar 17, 2010·Chemical Reviews·Seulki LeeXiaoyuan Chen
Oct 22, 2011·Molecular Pharmaceutics·Lei ZhuXiaoyuan Chen
Apr 16, 2009·Molecular Pharmaceutics·Emily Gullotti, Yoon Yeo
Aug 10, 2011·International Journal of Clinical Rheumatology·Daniel GolovkoHeike Daldrup-Link
May 23, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ji Young YheeIck Chan Kwon
Jan 5, 2010·PET Clinics·Gang Niu, Xiaoyuan Chen
Apr 28, 2012·Contrast Media & Molecular Imaging·Réjean LebelMartin Lepage
Jan 28, 2014·Biotechnology Journal·Hai-Yu HuCarsten Schultz
Apr 16, 2014·Medicinal Research Reviews·Laizhong ChenMinyong Li
Apr 5, 2014·Contrast Media & Molecular Imaging·Réjean Lebel, Martin Lepage
Apr 16, 2015·Contrast Media & Molecular Imaging·Sander M J van DuijnhovenHolger Grüll
Apr 10, 2012·Bioconjugate Chemistry·Anil WaghBenedict Law
Aug 31, 2012·Chembiochem : a European Journal of Chemical Biology·Anna Knapinska, Gregg B Fields
Aug 25, 2018·Chemical Society Reviews·Hong-Wen LiuWeihong Tan
Feb 11, 2011·Angewandte Chemie·Xin LinXiaoyuan Chen
Aug 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ling ZhangQihe Yin
Aug 29, 2020·Journal of Nanobiotechnology·Qiumei LanJinmin Zhao
Oct 27, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hwunjae LeeYoochan Hong
Nov 12, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shuyi Xiao, Liang Chen
Jan 15, 2011·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Sander M J van DuijnhovenHolger Grüll
Sep 17, 2009·Bioconjugate Chemistry·Benedict Law, Ching-Hsuan Tung
Jan 16, 2021·ACS Biomaterials Science & Engineering·Lucy Ngo, Melissa L Knothe Tate

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