PMID: 2478502Aug 1, 1989

Darkfield illumination improves microscopic detection of metals in Timm's stained tissue

The Histochemical Journal
E Baatrup, Christopher J Frederickson


Deposits of trace or toxic metals can be quickly identified by light microscopical surveys of tissue sections stained for metals by variants of Timm's silver enhancement method. The present work shows that the small, isolated silver grains that label isolated deposits of metal in tissue are undetectable in brightfield light microscopy but are easily detected in darkfield microscopy. Darkfield illumination is therefore recommended for improving the detection of trace or toxic metals in tissue.


May 7, 1990·Brain Research·G A Howell, Christopher J Frederickson
Feb 1, 1988·Toxicology and Applied Pharmacology·P M RodierR Simons
Mar 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Danscher, B Møller-Madsen
Jan 1, 1989·International Review of Neurobiology·Christopher J Frederickson
Oct 1, 1985·Toxicology and Applied Pharmacology·O Thorlacius-Ussing, G Danscher
Nov 8, 1985·The Journal of Comparative Neurology·F B Gaarskjaer
Sep 1, 1981·Experimental Neurology·Christopher J FredericksonM H Frederickson
Jan 1, 1984·International Review of Cytology·P Szerdahelyi, P Kása
May 14, 2005·Nature Reviews. Neuroscience·Christopher J FredericksonAshley I Bush


Apr 2, 2002·Experimental Neurology·Tony ValenteJ Pérez-Clausell
Oct 1, 1991·Environmental Research·J D SchiønningG Danscher

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