Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation

Data in Brief
A Peláez-GarcíaJ Ignacio Casal

Abstract

The transcription factor (TF) Snail1 is a major inducer of the epithelial-mesenchymal transition (EMT) during embryonic development and cancer progression. Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated their differentiation to osteoblasts or adipocytes. We used either stable isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out a proteomic analysis on the nuclear fraction since Snail is a nuclear TF that mediates its effects mainly through the regulation of other TFs. Proteomics data have been deposited in ProteomeXchange via the PRIDE partner repository with the dataset identifiers PXD001529 and PXD002157 (Vizcaino et al., 2014) [1]. Data are associated with a research article published in Molecular and Cellular Proteomics (Pelaez-Garcia et al., 2015) [2].

References

Oct 24, 2007·Nature Methods·Lukas KällMichael J MacCoss
Mar 18, 2008·Molecular & Cellular Proteomics : MCP·Hwee Tong TanMaxey C M Chung
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Dec 12, 2012·Molecular BioSystems·Yeon Hee BanYeo Joon Yoon
Apr 15, 2014·Nature Biotechnology·Juan A VizcaínoHenning Hermjakob

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Methods Mentioned

BETA
acetylation
electrophoresis

Software Mentioned

EasyProt
Proteome Discoverer
MSN
Perl script
extract
Phenyx
DAVID
Mascot

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