PMID: 9762664Oct 8, 1998Paper

Data on cytokine mRNA expression in CSF and peripheral blood mononuclear cells from MS patients as detected by PCR

Multiple Sclerosis : Clinical and Laboratory Research
P Monteyne, C J Sindic

Abstract

Reverse transcription polymerase chain reaction (RT-PCR) was used to amplify the mRNA coding for different cytokines in peripheral blood mononuclear cells (PBMC) and cerebrospinal fluid (CSF) cells from 18 multiple sclerosis (MS) patients as compared with 21 other neurological patients. mRNA levels were quantitated by radioactive hybridization of the PCR products. Expression of tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and interleukin (IL)-10 mRNA was elevated in CSF cells of MS patients. In many MS patients, both proinflammatory and immunoregulatory cytokine messages were detected in the CSF compartment. Such immune reactivity of CSF cells, as opposed to PBMC, was not associated with higher clinical activity of the disease. Expression of the B7.1 accessory molecule mRNA was similarly investigated. In the CSF, it was detected only in some clinically active MS cases and in other inflammatory diseases.

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Citations

Jun 22, 2010·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·D HesseF Sellebjerg
Dec 1, 1999·Annals of the Rheumatic Diseases·C LockL Steinman
Dec 14, 2004·Journal of Neuroimmunology·Isabelle MiyazawaYasuto Itoyama
May 13, 2011·Multiple Sclerosis : Clinical and Laboratory Research·Sabine LindquistMichael Sailer

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