Data on RT-qPCR assay of nuclear progesterone receptors (nPR), membrane progesterone receptors (mPR) and progesterone receptor membrane components (PGRMC) from human uterine endometrial tissue and cancer cells of the Uterine Cervix

Data in Brief
Natalia SmaglyukovaGeorg Sager

Abstract

A previous investigation showed that the endometrium normalized in women with endometrial hyperplasia after three months treatment with high dose levonorgestrel IUS (intrauterine system) [1] . The effect was maintained even if immunohistochemical analyses of the endometrium showed that nuclear progesterone receptors (nPRs) were completely downregulated. These observations indicated that some type of non-genomic effect existed [2]. We conducted new investigations of endometrial hyperplasia, now with 6 months low dose levonorgestrel IUS treatment. Again, the growth disturbances were reversed with normalization of the endometrium [3,4]. In the context of these studies, RT-qPCR analyses of the endometrium were performed before and after treatment, to determine expression of nuclear progesterone receptors (nPRA+B and nPRB), membrane progesterone receptors (mPR, α-, β- and γ-subtypes) and progesterone receptor membrane components (PGRMC1and PGRMC2). The human cervical cell line (C-4 I) [5] with no detectable nPRs [6,7] , was included in the investigation as biological control .The gene expression of nPRs, mPRs and PGRMCs was determined in the logarithmic growth phase. Tissue and cellular mRNA was determined with RT-qPCR and used as a...Continue Reading

References

Mar 22, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Georg SagerCatharina Engström
Jul 5, 2003·Physiological Reviews·Ralf M LoselMartin Wehling
Oct 22, 2008·Human Reproduction Update·B GellersenJ J Brosens
Jul 27, 2010·The Journal of Surgical Research·Marlin Wayne CauseyJonathan D Stallings

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Methods Mentioned

BETA
electrophoresis
biopsies
PCR
biopsy

Software Mentioned

Primer
geNorm
qBase
CFX Manager
blast
Primerdesign
geNorm V

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